Human dendritic cells as targets of dengue virus infection
| Autor: | John R. Mascola, Gerald S. Murphy, Curtis Hayes, Shuenn-Ju Wu, Sarah Schlesinger-Frankel, Mark K. Louder, Mary A. Marovich, Ravithat Putvatana, Deborah L. Birx, Timothy Burgess, Boonrat Tassaneetrithep, Wellington Sun, Michael A. Eller, Geraldine Grouard-Vogel |
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| Rok vydání: | 2002 |
| Předmět: |
Biopsy
Dermatology Dengue virus Biology In Vitro Techniques Immunofluorescence medicine.disease_cause Monocytes Dengue fever Pathogenesis medicine Humans Antibody-dependent enhancement Severe Dengue Molecular Biology Dengue vaccine Skin medicine.diagnostic_test Tumor Necrosis Factor-alpha Immune Sera Macrophages General Medicine Cell Biology Dendritic Cells Dengue Virus Exanthema medicine.disease Flow Cytometry Virology hemorrhagic virus infects APC Immunology Skin biopsy Tumor necrosis factor alpha Biotechnology |
| Zdroj: | The journal of investigative dermatology. Symposium proceedings. 6(3) |
| ISSN: | 1087-0024 |
| Popis: | Dengue virus infections are an emerging global threat. Severe dengue infection is manifested as dengue hemorrhagic fever and dengue shock syndrome, both of which can be fatal complications. Factors predisposing to complicated disease and pathogenesis of severe infections are discussed. Using immunohistochemistry, immunofluorescence, flow cytometry, and ELISA techniques, we studied the cellular targets of dengue virus infection, at both the clinical (in vivo) and the laboratory (in vitro) level. Resident skin dendritic cells are targets of dengue virus infection as demonstrated in a skin biopsy from a dengue vaccine recipient. We show that factors influencing infection of monocytes/macrophages and dendritic cells are different. Immature dendritic cells were found to be the cells most permissive for dengue infection and maybe early targets for infection. Immature dendritic cells exposed to dengue virus produce TNF-alpha protein. Some of these immature dendritic cells undergo TNF-alpha mediated maturation as a consequence of exposure to the dengue virus. |
| Databáze: | OpenAIRE |
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