Cholera toxin induced novel genes in human lymphocytes and monocytes
| Autor: | Marti Jett, Atabak R. Royaee, Linda Jong, David C.H. Yang, Chanaka Mendis, Rina Das |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cholera Toxin
Immunology CD2 Antigens Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Monocytes Adjuvanticity Gene expression medicine Humans Lymphocytes RNA Messenger Molecular Biology Gene Oligonucleotide Array Sequence Analysis Differential display Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cholera toxin Hypoxia-Inducible Factor 1 alpha Subunit Molecular biology CXCL2 Gene Expression Regulation Primer (molecular biology) |
| Zdroj: | Molecular Immunology. 43:1267-1274 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2005.07.008 |
| Popis: | Cholera toxin (CT) is well known as an inducer of the accumulation of cellular cAMP through the ADP-ribosylation of the Gs protein by CT. CT is also one of the most powerful mucosal adjuvants. However, the molecular mechanisms of the CT adjuvanticity are not well understood. Here, the transcriptional responses of cultured human lymphocytes and monocytes in response to CT were analyzed using differential display-PCR. The full complement of cellular mRNA was examined by high resolution polyarylamide gel electrophoresis and sequence analyses of the PCR products of 240 primer sets. Over 100 genes with altered expression were initially identified. The expressions of 65 of these genes were further analyzed and confirmed using custom glass cDNA arrays, RT-PCR and real-time PCR. Immunomodulatory genes such as CD2, HIF1, CXCL2, L-plastin, LILR and IFI30 were affected by CT. In addition, 14 novel genes with previously unknown functions were found to be CT induced. These CT induced gene expression alterations provide more insight in the mechanisms of CT actions. The CT induced gene expressions alterations could contribute to the CT adjuvanticity. |
| Databáze: | OpenAIRE |
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