Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed

Autor: Tomoo Sawabe, Tetsuya Hayashi, Shoko Endo, Yoshitoshi Ogura, Mami Tanaka, Sayaka Mino, Wataru Suda, Toshiyoshi Araki, Fumihito Kotake, Isao Yumoto, Hidetaka Doi, Masahira Hattori, Nurhidayu Al-saari, Toko Sawabe, Gao Feng, A.K.M. Rohul Amin
Rok vydání: 2017
Předmět:
0301 basic medicine
lcsh:Medicine
Biochemistry
Database and Informatics Methods
lcsh:Science
Clade
Data Management
Genetics
Multidisciplinary
biology
Phylogenetic tree
Phylogenetic Analysis
Genomics
Phylogenetics
Nucleic acids
Chemistry
Ribosomal RNA
Physical Sciences
Sequence Analysis
Research Article
Chemical Elements
Computer and Information Sciences
Cell biology
Cellular structures and organelles
Bioinformatics
Sequence analysis
030106 microbiology
Sequence Databases
Research and Analysis Methods
03 medical and health sciences
Evolutionary Systematics
Non-coding RNA
Vibrio
Taxonomy
Whole genome sequencing
Evolutionary Biology
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Computational Biology
Comparative Genomics
Genome Analysis
biology.organism_classification
16S ribosomal RNA
Biological Databases
030104 developmental biology
RNA
lcsh:Q
Ribosomes
Hydrogen
Zdroj: PLoS ONE, Vol 12, Iss 6, p e0180053 (2017)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0180053
Popis: A novel strain Vibrio aphrogenes sp. nov. strain CA-1004(T) isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using poly phasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis Glade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis Glade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004(T) as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004(T) was separate from four known Rumoiensis Glade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were non motile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis Glade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis Glade, with CA-1004(T) as the type strain (JCM 31643(T) = DSM 103759(T)).
Databáze: OpenAIRE
Externí odkaz: