Genome-wide analysis of DNA methylation in rat lungs with lipopolysaccharide-induced acute lung injury
| Autor: | Huan‑Huan Tian, Jing Qin, Xiao‑Zhi Wang, Xiang‑Yong Liu, Xiao‑Qiang Zhang, Chang Jun Lv, Yan Li, Dong Hao |
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| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male Cancer Research Acute Lung Injury Biology Lung injury Biochemistry law.invention Rats Sprague-Dawley law Genetics Animals Humans Methylated DNA immunoprecipitation Promoter Regions Genetic Lung Molecular Biology Gene Genetic Association Studies Polymerase chain reaction Oligonucleotide Array Sequence Analysis Respiratory Distress Syndrome Genome Reverse Transcriptase Polymerase Chain Reaction Reproducibility of Results Molecular Sequence Annotation Promoter Methylation DNA Methylation respiratory system Chromosomes Mammalian Rats respiratory tract diseases Oncology CpG site DNA methylation Cancer research Molecular Medicine CpG Islands |
| Zdroj: | Molecular Medicine Reports. 7:1417-1424 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2013.1405 |
| Popis: | Acute lung injury and acute respiratory distress syndrome (ALI/ARDS) are associated with high morbidity and mortality in patients, however, the precise pathogenesis of ALI/ARDS remains unknown. Lipopolysaccharide (LPS) exhibits a number of critical functions and may be associated with the DNA methylation of genes in the lungs. In the present study a genome‑wide analysis of DNA methylation was performed in rat lungs with LPS‑induced ALI/ARDS. Normal and LPS‑induced lung tissues with ALI were analyzed using methylated DNA immunoprecipitation and a rat DNA methylation promoter plus CpG island microarray and the candidate genes were validated by quantitative reverse transcriptase polymerase chain reaction (qRT‑PCR). Aberrant DNA methylation of the promoter regions of 1,721 genes and the CpG islands of 990 genes was identified when normal lung tissues and lung tissues with LPS‑induced ALI/ARDS were compared. These genes were commonly located on chromosomes 1, 3, 5, 7 and 10 (P |
| Databáze: | OpenAIRE |
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