Single synaptic observation of cholinergic neurotransmission on living neurons: concentration and dynamics
| Autor: | Justin DesLaurier, Ran Chen, Theresa M. Welle, Jonathan V. Sweedler, Mei Shen, Zizheng Qu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
In situ
Cholinergic Agents 02 engineering and technology Neurotransmission 010402 general chemistry 01 natural sciences Biochemistry Synaptic Transmission Catalysis Article Synapse chemistry.chemical_compound Scanning electrochemical microscopy Colloid and Surface Chemistry Aplysia medicine Animals Neurotransmitter Neurons biology General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences chemistry Biophysics Cholinergic Microscopy Electrochemical Scanning 0210 nano-technology Acetylcholine medicine.drug |
| Popis: | Acetylcholine, the first neurotransmitter identified more than a century ago, plays critical roles in human activities and health; however, its synaptic concentration dynamics have remained unknown. Here, we demonstrate the in situ simultaneous measurements of synaptic cholinergic transmitter concentration and release dynamics. We used nanoscale electroanalytical methods: nanoITIES electrode of 15 nm in radius and nano-resolved scanning electrochemical microscopy (SECM). Time-resolved in situ measurements unveiled information on synaptic acetylcholine concentration and release dynamics of living Aplysia neurons. The measuring technique enabled the quantitative sensing of acetylcholine with negligible interference of other ionic and redox-active species. We measured cholinergic transmitter concentrations very close to the synapse, with values as high as 2.4 mM. We observed diverse synaptic transmitter concentration dynamics consisting of singlet, doublet and multiplet events with a signal to noise ratio of 6 to 130. The unprecedented details about synaptic neurotransmission unveiled are instrumental for understanding brain communication and diseases in a way distinctive from extra-synaptic studies. |
| Databáze: | OpenAIRE |
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