Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells
| Autor: | D.H. Gardner, Leanne Thomas, Lindsay Marshall, D.M. Goodall, Elizabeth E. Torr, Helen R. Griffiths, Rachel S. Willetts, Anne Bielemeier, Andrew Devitt |
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| Rok vydání: | 2011 |
| Předmět: |
ICAM-3
Phagocytosis Cell Inflammation Biology Jurkat cells Apoptotic cell clearance Antibodies Monoclonal Murine-Derived Jurkat Cells Mice 03 medical and health sciences 0302 clinical medicine Antigens CD Cell-Derived Microparticles medicine Animals Humans Macrophage Molecular Biology 030304 developmental biology Mice Inbred BALB C Original Paper 0303 health sciences Chemotaxis Macrophages apoptosis phagocytosis Cell Biology Intercellular adhesion molecule Cell biology medicine.anatomical_structure inflammation monocytes/macrophages 030220 oncology & carcinogenesis medicine.symptom Cell Adhesion Molecules HeLa Cells |
| Zdroj: | Cell Death and Differentiation |
| ISSN: | 1476-5403 1350-9047 |
| DOI: | 10.1038/cdd.2011.167 |
| Popis: | A wide range of molecules acting as apoptotic cell-associated ligands, phagocyte-associated receptors or soluble bridging molecules have been implicated within the complex sequential processes that result in phagocytosis and degradation of apoptotic cells. Intercellular adhesion molecule 3 (ICAM-3, also known as CD50), a human leukocyte-restricted immunoglobulin super-family (IgSF) member, has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined. The main objective of this work is to further characterise the function of ICAM-3 in the removal of apoptotic cells. Using a range of novel anti-ICAM-3 monoclonal antibodies (mAbs), including one (MA4) that blocks apoptotic cell clearance by macrophages, alongside apoptotic human leukocytes that are normal or deficient for ICAM-3, we demonstrate that ICAM-3 promotes a domain 1-2-dependent tethering interaction with phagocytes. Furthermore, we demonstrate an apoptosis-associated reduction in ICAM-3 that results from release of ICAM-3 within microparticles that potently attract macrophages to apoptotic cells. Taken together, these data suggest that apoptotic cell-derived microparticles bearing ICAM-3 promote macrophage chemoattraction to sites of leukocyte cell death and that ICAM-3 mediates subsequent cell corpse tethering to macrophages. The defined function of ICAM-3 in these processes and profound defect in chemotaxis noted to ICAM-3-deficient microparticles suggest that ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. |
| Databáze: | OpenAIRE |
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