Overcoming analytical challenges to generate data critical to understanding lipid nanoparticle-delivered modified mRNA biodistribution
| Autor: | Glen Hawthorne, Mikko Hölttä, Amanda Wilson, Sima Khan, Johnny Lindqvist, Neil Henderson |
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| Rok vydání: | 2019 |
| Předmět: |
Drug Carriers
0303 health sciences Analyte Messenger RNA Biodistribution Bioanalysis Computer science Clinical Biochemistry food and beverages General Medicine Computational biology Lipids Analytical Chemistry Mice 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine 030220 oncology & carcinogenesis Animals Nanoparticles Tissue Distribution RNA Messenger Sample collection General Pharmacology Toxicology and Pharmaceutics 030304 developmental biology |
| Zdroj: | Bioanalysis. 11:1993-2001 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Aim: Chemically modified mRNA offers a novel approach to treat disease. Due to susceptibility to extracellular nucleases in vivo, dosed modified mRNA therapeutics can benefit from encapsulation within novel delivery systems, such as lipid nanoparticles (LNPs). To understand the holistic effect of dosing LNP-encapsulated modified mRNA therapeutics can require bioanalysis of several components including the mRNA, protein and LNP. Methodology: These components can require bespoke preanalytical strategies to preserve analyte integrity to achieve successful analysis. Here we describe the sample collection, processing steps and bioanalytical technologies that can be used to overcome these challenges. Discussion: Understanding the biodistribution and holistic effects of the different components allow the pharmaceutical industry to evaluate safety and efficacy of modified mRNA therapeutics. |
| Databáze: | OpenAIRE |
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