Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis
| Autor: | M. A. W. G. Van Der Hoorn, W. J. Terpstra, Claude Yersin, Rudy A. Hartskeerl, Henk L. Smits, Marga G. A. Goris, G. C. Gussenhoven, D. M. Sasaki |
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| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Seychelles Sensitivity and Specificity Hawaii Microbiology Serology Blood serum Leptospira Reference Values Direct agglutination test Medicine Humans Leptospirosis Netherlands Latex beads biology business.industry Reproducibility of Results Bacteriology medicine.disease biology.organism_classification Antibodies Bacterial Latex fixation test Agglutination (biology) business Latex Fixation Tests |
| Zdroj: | Journal of clinical microbiology. 38(3) |
| ISSN: | 0095-1137 |
| Popis: | A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agglutination test was evaluated with groups of serum samples from patients with leptospirosis and control patients from Hawaii, the Seychelles, Thailand, and The Netherlands. The mean overall sensitivity was 82.3%, and the mean overall specificity was 94.6%. The assay is easy to perform and does not require special skills or equipment. The reagents have a long shelf life, even at tropical temperatures. Together, these factors make the assay suitable for use even at the peripheral level of a health care system as a rapid screening test for leptospirosis. |
| Databáze: | OpenAIRE |
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