Electrophysiological and Behavioral Responses of a Cuban Population of the Sweet Potato Weevil to its Sex Pheromone

Autor: Angel Guerrero, Tania Sureda, Carmen Quero, Michel Renou, María Pilar Bosch, Rubén Avilés, Francisco Coll
Přispěvatelé: Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Instituto de Investigaciones Fundamentales en Agricultura Tropical 'Alejandro de Humboldt', Partenaires INRAE, Universidad de La Habana [Cuba], Unité de phytopharmacie et médiateurs chimiques, Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2006
Předmět:
Male
0106 biological sciences
Chromatography
Gas
Time Factors
Stereochemistry
Population
SYNTHESIS
010603 evolutionary biology
01 natural sciences
Biochemistry
Mass Spectrometry
SWEET POTATO WEEVIL
OLFACTOMETER BIOASSAY
ELECTROPHYSIOLOGICAL ACTIVITY
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Botany
Animals
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Ipomoea batatas
Sex Attractants
education
Solid Phase Microextraction
Ecology
Evolution
Behavior and Systematics
education.field_of_study
IDENTIFICATION
Behavior
Animal
Dose-Response Relationship
Drug
Esterification
biology
Weevil
Animal Structures
Cuba
General Medicine
FIELD TESTS
biology.organism_classification
Attraction
CYLAS FORMICARIUS ELEGANTULUS
Electrophysiology
010602 entomology
Olfactometer
Sex pheromone
SEX PHEROMONE
Weevils
Pheromone
Female
sense organs
PEST analysis
Convolvulaceae
Zdroj: Journal of Chemical Ecology
Journal of Chemical Ecology, Springer Verlag, 2006, 32 (10), pp.2177-2190. ⟨10.1007/s10886-006-9138-4⟩
ISSN: 1573-1561
0098-0331
DOI: 10.1007/s10886-006-9138-4
Popis: International audience; The sex pheromone of feral sweet potato weevils Cylas formicarius elegantulus from Cuba was found, via solid-phase microextraction analysis, to be identical to (Z)-3-dodecenyl (E)-2-butenoate, a previously reported compound. Females emitted 20 pg pheromone d−1. In scanning electron microscopy studies carried out on the male antenna, we identified several types of sensilla: sensilla trichoidea of type 1 (ST1) as long hairs (100–150 μm), sensilla trichoidea of type 2 as short hairs (50–60 μm), sensilla basiconica of type 1 as thick pegs (20–25 μm), sensilla basiconica of type 2 as curved pegs (10–15 μm), and sensilla basiconica of type 3 as thin and straight short pegs (15–20 μm). The same types were observed in female antennae but ST1 were far less abundant than in males. Sensilla chaetica were also found on the flagellum subsegments in both sexes. In electrophysiological tests, the crotonate function in the pheromone structure proved to be critical for activity since regular depolarizations (0.6–0.8 mV) were obtained with puffs on 1 μg of the attractant, but not with puffs of the formate, acetate, propionate, or butyrate analogue of the pheromone. In a double dual-choice olfactometer, males showed maximum activity between the 4th and the 8th hr of scotophase at a dose of 50–1000 ng of pheromone. In field tests, a correlation between the contents of the Z,E isomer in the pheromone formulation with activity was noticed, and baits containing this isomer of stereomeric purity >94% showed the highest attractivity. The presence of 5% of the Z,Z isomer in the lure did not induce any synergistic or inhibitory effect, and the alcohol precursor of the pheromone was inactive. The results show that use of a stereomerically pure pheromone may not be necessary in pest control strategies.
Databáze: OpenAIRE
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