Type V collagen fibrils in mouse metanephroi
Autor: | Pi-Chao Wang, Yusuke Murasawa, Yusuke Nishimura, Pan Qi, Han-Hsiu Hsu |
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Rok vydání: | 2013 |
Předmět: |
Kidney Glomerulus
Biophysics Morphogenesis Nephrogenesis macromolecular substances Kidney Fibril Biochemistry Collagen Type I Extracellular matrix Mice Animals Molecular Biology Mice Inbred ICR Ureteric bud Chemistry Metanephric kidney Kidney metabolism Cell Biology Anatomy Type V collagen In vitro Cell biology Cellular Microenvironment Female Wound healing Collagen Type V Type I collagen |
Zdroj: | Biochemical and Biophysical Research Communications. 441:649-654 |
ISSN: | 0006-291X |
Popis: | Type V collagen (Col V) molecule, a minor component of kidney connective tissues, was found in adult cornea, and has been considered as a regulatory fibril-forming collagen that emerges into type I collagen to trigger the initiation of Col I fiber assembly. Col V was also found in injured, wound healing tissues or placenta, and was considered as a dysfunctional extracellular matrix (ECM). Reconstituted Col V fibril was characterized as an ECM to detach cells in vitro, and our previous study showed that the reconstituted Col V fibril facilitated the migration of glomerular endothelial cells and induced ECM remodeling, whereas Col V molecules stabilized cells. These facts suggest that not only the structure but also the function of Col V fibril are different from Col V molecule. Recently, Col V molecule has been reported existing in various developing tissues such as bone and lung, but Col V fibril has not been reported yet. In this study, we firstly explored the existence of Col V fibril in metanephroi, and found it distributed in the immature kidney tissues whereas disappeared when the tissues reached mature. It is likely that Col V fibril may form a prototype of pericellular microenvironment and the transient existence of Col V fibril may play a role as the pioneering ECM during metanephric tissue morphogenesis. |
Databáze: | OpenAIRE |
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