rhBMP-2 Pre-Treated Human Periodontal Ligament Stem Cell Sheets Regenerate a Mineralized Layer Mimicking Dental Cementum

Autor: Joo-Young Park, Jeong-Ho Yun, Takanori Iwata, Chan Ho Park, Si-Na Kim, TacGhee Yi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Mineralized tissues
Bone Morphogenetic Protein 2
stem cell therapy
lcsh:Chemistry
Mice
0302 clinical medicine
Tissue engineering
lcsh:QH301-705.5
Cells
Cultured
Spectroscopy
mesenchymal stem cell
Dental Cementum
Mice
Inbred BALB C
Tissue Scaffolds
Chemistry
General Medicine
Recombinant Proteins
Computer Science Applications
medicine.anatomical_structure
tissue engineering
Ligament
Hydroxyapatites
Periodontal Ligament
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
stomatognathic system
medicine
Animals
Humans
Periodontal fiber
dental stem cells
Cementum
Physical and Theoretical Chemistry
Molecular Biology
Dental alveolus
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
030206 dentistry
stomatognathic diseases
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
regeneration
Dental cementum
Stem Cell Transplantation
Biomedical engineering
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 3767, p 3767 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 11
ISSN: 1661-6596
1422-0067
Popis: The periodontal complex consisting of alveolar bone, cementum, and periodontal ligaments (PDL) supports human teeth through the systematic orchestration of mineralized tissues and fibrous tissues. Importantly, cementum, the outermost mineralized layer of dental roots, plays an essential role by bridging the inner ligaments from the dental root to the alveolar bone. When the periodontal complex is damaged, the regeneration of each component of the periodontal complex is necessary
however, it is still challenging to achieve complete functional regeneration. In this study, we tried to control the regeneration of cementum and PDL by using a human PDL stem cell (hPDLSC) sheet engineering technology with the pretreatment of recombinant human BMP-2 (rhBMP-2). Isolated hPDLSCs obtained from extracted human teeth were pretreated with rhBMP-2 for in vitro osteogenic differentiation and grafted on the micro/macro-porous biphasic calcium phosphate (MBCP) blocks, which represent dental roots. The MBCPs with hPDLSC sheets were implanted in the subcutaneous layer of immune-compromised mice, and rhBMP-2 pretreated hPDLSC sheets showed higher mineralization and collagen ligament deposition than the no-pretreatment group. Therefore, the rhBMP-2-hPDLSC sheet technique could be an effective strategy for the synchronized regeneration of two different tissues: mineralized tissue and fibrous tissues in periodontal complexes.
Databáze: OpenAIRE
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