Metformin promotes susceptibility to experimental Leishmania braziliensis infection
| Autor: | Lais de Melo Ferreira, Edgar Marcelino de Carvalho Filho, Icaro Bonyek-Silva, Tainá Alves Malta, Sérgio Arruda, Reinan Lima Santos, Natalia Machado Tavares, Filipe R Lima |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
RC955-962 030231 tropical medicine Leishmaniasis Cutaneous Biology immunomodulation Microbiology Parasite load Leishmania braziliensis susceptibility Lesion 03 medical and health sciences Mice cutaneous leishmaniasis 0302 clinical medicine Cutaneous leishmaniasis Arctic medicine. Tropical medicine medicine Parasite hosting Animals Leishmania Mice Inbred BALB C Leishmaniasis medicine.disease biology.organism_classification QR1-502 Metformin infection Original Article medicine.symptom metformin Macrophage proliferation Brazil medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 115, Article number: e200272, Published: 16 NOV 2020 Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 115 (2020) |
| Popis: | BACKGROUND Metformin (MET) is a hypoglycemic drug used for the treatment of diabetes, despite interference in host immunity against microorganisms. Cutaneous infection caused by pathogens such as Leishmania braziliensis (Lb), the agent responsible for cutaneous leishmaniasis (CL) in Brazil, represents an interesting model in which to evaluate the effects associated with MET. OBJECTIVE To evaluate the modulatory effect of MET in Lb infection. MATERIAL AND METHODS Experimental study of Lb infection and MET treatment in BALB/c mice and Raw 264.7 macrophages. FINDINGS MET treatment interfered with lesion kinetics, increased parasite load and reduced macrophage proliferation. Low concentrations of MET in Lb culture allow for the maintenance of stationary parasite growth phase. Lb-infected cells treated with MET exhibited increased parasite load. While both MET and Lb infection alone promoted the production of intracellular reactive oxygen species (ROS), reduced levels of ROS were seen in MET-treated Lb-infected macrophages. MAIN CONCLUSION Experimental treatment with MET interfered with the kinetics of cutaneous ulceration, increased Lb parasite load, altered ROS production and modulated cellular proliferation. Our experimental results indicate that MET interfere with the evolution of CL. |
| Databáze: | OpenAIRE |
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