Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats
| Autor: | Lilla Tar, Árpád Párdutz, Annamária Fejes-Szabó, Zsuzsanna Bohár, Klaudia Flóra Laborc, Eleonóra Spekker, Gábor Nagy-Grócz, László Vécsei |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.drug_class Estrogen receptor trigeminal system c-Fos Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine pain Journal of Pain Research Original Research biology business.industry Cholesterol Spinal trigeminal nucleus Sexual dimorphism 030104 developmental biology Anesthesiology and Pain Medicine Endocrinology medicine.anatomical_structure chemistry Estrogen sexual dimorphism biology.protein Ovariectomized rat business headache 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Pain Research |
| ISSN: | 1178-7090 |
| Popis: | Annamária Fejes-Szabó,1 Eleonóra Spekker,2 Lilla Tar,3 Gábor Nagy-Grócz,1,4 Zsuzsanna Bohár,1,2 Klaudia Flóra Laborc,2,5 László Vécsei,1,2 Árpád Párdutz2 1MTA-SZTE Neuroscience Research Group, Szeged, Hungary; 2Department of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Centre, University of Szeged, Szeged, Hungary; 3Department of Neurology, University of Ulm, Ulm, Germany; 4Faculty of Health Sciences and Social Studies, University of Szeged, Szeged, Hungary; 5Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI, USA Background: The prevalence of craniofacial pain disorders show sexual dimorphism with generally more common appearance in women suggesting the influence of estradiol, but the exact cause remains unknown. The common point in the pathogenesis of these disorders is the activation of trigeminal system. One of the animal experimental models of trigeminal activation is the orofacial formalin test, in which we investigated the effect of chronic 17β-estradiol pretreatment on the trigeminal pain-related behavior and activation of trigeminal second-order neurons at the level of spinal trigeminal nucleus pars caudalis (TNC). Methods: Female Sprague Dawley rats were ovariectomized and silicone capsules were implanted subcutaneously containing cholesterol in the OVX group and 17β-estradiol and cholesterol in 1:1 ratio in the OVX+E2 group. We determined 17β-estradiol levels in serum after the implantation of capsules. Three weeks after operation, 50 µL of physiological saline or 1.5% of formalin solution was injected subcutaneously into the right whisker pad of rats. The time spent on rubbing directed to the injected area and c-Fos immunoreactivity in TNC was measured as the formalin-induced pain-related behavior, and as the marker of pain-related neuronal activation, respectively. Results: The chronic 17β-estradiol pretreatment mimics the plasma levels of estrogen occurring in the proestrus phase and significantly increased the formalin-induced pain-related behavior and neuronal activation in TNC. Conclusion: Our results demonstrate that the chronic 17β-estradiol treatment has strong pronociceptive effect on orofacial formalin-induced inflammatory pain suggesting modulatory action of estradiol on head pain through estrogen receptors, which are present in the trigeminal system. Keywords: c-Fos, headache, pain, sexual dimorphism, trigeminal system |
| Databáze: | OpenAIRE |
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