Molecular Methods for the Epidemiological Typing of Salmonella enterica Serotype Typhi from Hong Kong and Vietnam
| Autor: | Y. M. Ho, A. F. Cheng, J. M. Ling, Le Thi Phi, K. M. Kam, Nguyen Thi Tuyet Hoa, Norman Lo |
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| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Serotype Time Factors Microbial Sensitivity Tests Salmonella typhi DNA Ribosomal Typhoid fever Microbiology Ribotyping medicine Typing Serotyping Typhoid Fever Deoxyribonucleases Type II Site-Specific Bacteriophage Typing Phage typing Molecular Epidemiology Molecular epidemiology biology Reproducibility of Results Bacteriology Drug Resistance Microbial biology.organism_classification medicine.disease DNA Fingerprinting Virology Bacterial Typing Techniques Vietnam Salmonella enterica Hong Kong Plasmids |
| Zdroj: | Journal of Clinical Microbiology. 38:292-300 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.38.1.292-300.2000 |
| Popis: | A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with Pst I, and total DNA fingerprinting with Nar I. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi. |
| Databáze: | OpenAIRE |
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