Haplotype CGC from XPD, hOGG1 and ITGA2 polymorphisms increases the risk of nasopharyngeal carcinoma in Malaysia
| Autor: | Yoke-Yeow Yap, Pei Pei Chong, Hejar Abdul Rahman, Siew Ying Crystale Lim, Eng-Zhuan Ban, Munn-Sann Lye |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Linkage disequilibrium Integrins Heredity Integrin alpha2 lcsh:Medicine Artificial Gene Amplification and Extension Restriction Fragment Mapping medicine.disease_cause Polymerase Chain Reaction Linkage Disequilibrium DNA Glycosylases Habits 0302 clinical medicine Gene Frequency Risk Factors Genotype Medicine and Health Sciences Smoking Habits lcsh:Science Genetics Multidisciplinary Nasopharyngeal Carcinoma Alcohol Consumption Middle Aged Extracellular Matrix Genetic Mapping Oncology 030220 oncology & carcinogenesis Female Restriction fragment length polymorphism Cellular Structures and Organelles Research Article Restriction Fragment Length Polymorphism Analysis Variant Genotypes Biology Research and Analysis Methods Carcinomas Polymorphism Single Nucleotide 03 medical and health sciences medicine Cell Adhesion Humans Genetic Predisposition to Disease Allele Molecular Biology Techniques Molecular Biology Genetic Association Studies Nutrition Xeroderma Pigmentosum Group D Protein Behavior lcsh:R Haplotype Gene Mapping Carcinoma Malaysia Cancers and Neoplasms Biology and Life Sciences Nasopharyngeal Neoplasms Cell Biology medicine.disease Diet 030104 developmental biology Nasopharyngeal carcinoma Haplotypes Cancer research lcsh:Q Carcinogenesis Nucleotide excision repair |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 11, p e0187200 (2017) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND:8-oxoG, a common DNA lesion resulting from reactive oxygen species (ROS), has been shown to be associated with cancer initiation. hOGG1 DNA glycosylase is the primary enzyme responsible for excision of 8-oxoG through base excision repair (BER). Integrins are members of a family of cell surface receptors that mediate the cell-cell and extracellular matrix (ECM) interactions. Integrins are involved in almost every aspect of carcinogenesis, from cell differentiation, cell proliferation, metastasis to angiogenesis. Loss of ITGA2 expression was associated with enhanced tumor intravasation and metastasis of breast and colon cancer. XPD gene encodes DNA helicase enzyme that is involved in nucleotide excision repair (NER). It is shown in previous research that XPD homozygous wildtype Lys/Lys genotype was associated with higher odds of NPC. METHODS:We conducted a 1 to N case-control study involving 300 nasopharyngeal carcinoma (NPC) cases and 533 controls matched by age, gender and ethnicity to investigate the effect of hOGG1 Ser326Cys, ITGA2 C807T and XPD Lys751Gln polymorphisms on NPC risk. Linkage disequilibrium and haplotype analysis were conducted to explore the association of allele combinations with NPC risk. Restriction fragment length polymorphism (RFLP-PCR) was used for DNA genotyping. RESULTS:No significant association was observed between hOGG1 Ser326Cys and ITGA2 C807T polymorphisms with NPC risk after adjustment for age, gender, ethnicity, cigarette smoking, alcohol and salted fish consumption. Lys/Lys genotype of XPD Lys751Gln polymorphism was associated with increased NPC risk (OR = 1.60, 95% CI = 1.06-2.43). Subjects with history of smoking (OR = 1.81, 95% CI = 1.26-2.60), and salted fish consumption before age of 10 (OR = 1.77, 95% CI = 1.30-2.42) were observed to have increased odds of NPC. The odds of developing NPC of CGC haplotype was significantly higher compared to reference AGC haplotype (OR = 2.20, 95% CI = 1.06-4.58). CONCLUSION:The allele combination of CGC from hOGG1, ITGA2 and XPD polymorphisms was significantly associated with increased odds of NPC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|