Regulation of bone marrow stromal cell differentiation by cytokines whose receptors share the gp130 Protein
| Autor: | Xiying Wu, Molly R. Hill, Helen B. Bass, Katherine A. Kelly, Jeffrey M. Gimble, Frank Wanker, Chi-Sun Wang, George D. Yancopoulos |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Stromal cell
Hydrocortisone Bone Marrow Cells Nerve Tissue Proteins Oncostatin M Leukemia Inhibitory Factor Biochemistry Cell Line Mice Interleukin 20 Antigens CD Adipocytes Cytokine Receptor gp130 Animals Humans Ciliary Neurotrophic Factor Receptors Cytokine Interleukin 6 Genes Immediate-Early Molecular Biology Common gamma chain Lymphokines Membrane Glycoproteins Osteoblasts biology Interleukin-6 Interleukins Cell Differentiation Cell Biology Glycoprotein 130 Growth Inhibitors Rats Cell biology Lipoprotein Lipase biology.protein Cancer research Cytokines Ciliary neurotrophic factor receptor Stromal Cells Peptides Leukemia inhibitory factor Signal Transduction |
| Zdroj: | Journal of Cellular Biochemistry. 54:122-133 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.240540113 |
| Popis: | The bone marrow stroma consists of a heterogeneous population of cells which participate in osteogenic, adipogenic, and hematopoietic events. The murine stromal cell line, BMS2, exhibits the adipocytic and osteoblastic phenotypes in vitro. BMS2 differentiation was examined in response to cytokines which share the gp130 signal transducing protein within their receptor complex. Four of the cytokines (interleukin 6, interleukin 11, leukemia inhibitory factor, and oncostatin M) inhibited hydrocortisone-induced adipocyte differentiation in a dose dependent manner based on lipid accumulation and lipoprotein lipase enzyme activity. Inhibition occurred only when the cytokines were present during the initial 24 h of the induction period; after 48 h their effects were diminished. Likewise, these cytokines increased alkaline phosphatase enzyme activity twofold in preadipocyte BMS2 cells. Both leukemia inhibitory factor and oncostatin M induced early active gene expression in resting preadipocyte BMS2 cells and decreased the steady state mRNA level of a unique osteoblastic gene marker, osteocalcin. A fifth cytokine whose receptor complex shares the gp130 protein, ciliary neurotrophic factor, did not significantly regulate stromal cell differentiation when added by itself. However, with the addition of a missing component of its receptor complex, ciliary neurotrophic factor receptor alpha protein, this cytokine also inhibited BMS2 adipogenesis. Together, these data indicate that the cytokines whose receptors share the gp130 protein can modulate stromal cell commitment to the adipocyte and osteoblast differentiation pathways. |
| Databáze: | OpenAIRE |
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