Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Autor: Maria G. E. G. Bremer, Georgina M. S. Ross, Michel W. F. Nielen, Aart van Amerongen, Jan H. Wichers
Jazyk: angličtina
Předmět:
Carbon nanoparticles
Carbon Nanoparticles
Monsteradministratie & Coördinatie
lcsh:Biotechnology
Clinical Biochemistry
02 engineering and technology
01 natural sciences
Article
BU Dierbehandelingsmiddelen
BU Veterinary Drugs
Matrix (chemical analysis)
Corylus
Limit of Detection
lcsh:TP248.13-248.65
Surface plasmon resonance
Humans
Food allergens
VLAG
Immunoassay
Chromatography
Smartphone detection
biology
Chemistry
Ligand binding assay
010401 analytical chemistry
Organic Chemistry
Antibodies
Monoclonal
General Medicine
High-speed lateral flow immunoassay
Antigens
Plant
021001 nanoscience & nanotechnology
Antibody selection
Organische Chemie
Carbon
0104 chemical sciences
Test line
BBP Bioconversion
Smartphone app
biology.protein
Nanoparticles
Food allergen
Antibody
0210 nano-technology
Food Hypersensitivity
Zdroj: Biosensors, 8(4)
Biosensors
Volume 8
Issue 4
Biosensors, Vol 8, Iss 4, p 130 (2018)
Biosensors 8 (2018) 4
ISSN: 2079-6374
DOI: 10.3390/bios8040130
Popis: Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.
Databáze: OpenAIRE
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