Nerve growth factor promoter activity revealed in mice expressing enhanced green fluorescent protein
| Autor: | Janet Elliott, Bernadeta Michalski, Michael D. Kawaja, Gina Trinh, Margaret Fahnestock, Laura J. Smithson, Anne-Marie Crotty |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Genetically modified mouse Transgene Green Fluorescent Proteins Population Mice Transgenic Biology Article Green fluorescent protein Mice Genes Reporter Reticular cell Nerve Growth Factor Animals Tissue Distribution RNA Messenger Transgenes Promoter Regions Genetic education education.field_of_study General Neuroscience Brain Sciatic Nerve Molecular biology Nerve growth factor nervous system biology.protein Female Sciatic nerve Neurotrophin |
| Zdroj: | The Journal of Comparative Neurology. 519:2522-2545 |
| ISSN: | 0021-9967 |
| Popis: | Nerve growth factor (NGF) and its precursor proNGF are perhaps the best described growth factors of the mammalian nervous system. There remains, however, a paucity of information regarding the precise cellular sites of proNGF/NGF synthesis. Here we report the generation of transgenic mice in which the NGF promoter controls the ectopic synthesis of enhanced green fluorescent protein (EGFP). These transgenic mice provide an unprecedented resolution of both neural cells (e.g., neocortical and hippocampal neurons) and non-neural cells (e.g., renal interstitial cells and thymic reticular cells) that display NGF promoter activity from postnatal development to adulthood. Moreover, the transgene is inducible by injury. At 2 days after sciatic nerve ligation, a robust population of EGFP-positive cells is seen in the proximal nerve stump. These transgenic mice offer novel insights into the cellular sites of NGF promoter activity and can be used as models for investigating the regulation of proNGF/NGF expression after injury. |
| Databáze: | OpenAIRE |
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