Poly(ADP-ribosyl)ation as a new posttranslational modification of YB-1

Autor: Elizaveta E. Alemasova, Nina A. Moor, Lev P. Ovchinnikov, Maria V. Sukhanova, Dmitry A. Kretov, P. E. Pestryakov, Olga I. Lavrik, Patrick A. Curmi
Přispěvatelé: Institute of Chemical Biology and Fundamental Medicine [Novosibirsk, Russia] (ICBFM SB RAS), Siberian Branch of the Russian Academy of Sciences (SB RAS), Université d'État de Novossibirsk, Institute of Protein Research, Russian Academy of Sciences, Pushchino, Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Maciejak, Olek
Rok vydání: 2015
Předmět:
Poly Adenosine Diphosphate Ribose
Saccharomyces cerevisiae Proteins
HMG-box
DNA Repair
DNA repair
DNA polymerase
Poly ADP ribose polymerase
DNA polymerase II
[SDV]Life Sciences [q-bio]
Y-box binding protein 1 (YB-1)
Poly (ADP-Ribose) Polymerase-1
Electrophoretic Mobility Shift Assay
Biochemistry
Models
Biological
Humans
DNA Breaks
Double-Stranded
AP endonuclease 1 (APE1)
DNA clamp
biology
Multiple DNA lesions
General Medicine
Base excision repair
DNA
NAD
Peptide Fragments
Recombinant Proteins
Proliferating cell nuclear antigen
Up-Regulation
[SDV] Life Sciences [q-bio]
Isoenzymes
Kinetics
Oxidative Stress
Poly(ADP-ribose)polymerase 1 (PARP1)
Mutation
biology.protein
pADPr)
Y-Box-Binding Protein 1
Poly(ADP-ribose) (PAR
Poly(ADP-ribose) Polymerases
Protein Processing
Post-Translational
DNA Damage
Zdroj: Biochimie
Biochimie, Elsevier, 2015, 119, pp.36-44. ⟨10.1016/j.biochi.2015.10.008⟩
Biochimie, 2015, 119, pp.36-44. ⟨10.1016/j.biochi.2015.10.008⟩
ISSN: 1638-6183
0300-9084
Popis: International audience; Multifunctional Y-box binding protein 1 (YB-1) is actively studied as one of the components of cellular response to genotoxic stress. However, the precise role of YB-1 in the process of DNA repair is still obscure. In the present work we report for the first time new posttranslational modification of YB-1 - poly(ADP-ribosyl)ation, catalyzed by one of the main regulatory enzymes of DNA repair - poly(ADP-ribose)polymerase 1 (PARP1) in the presence of model DNA substrate carrying multiple DNA lesions. Therefore, poly(ADP-ribosyl)ation of YB-1 catalyzed with PARP1, can be stimulated by damaged DNA. The observed property of YB-1 underlines its ability to participate in the DNA repair by its involvement in the regulatory cascades of DNA repair.
Databáze: OpenAIRE
Externí odkaz: