Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients
| Autor: | Polyanna Miranda Alves, Lusânia Maria Greggi Antunes, Francisca da Luz Dias, Paulo Roberto Juliano Martins, Rommel Rodríguez Burbano, Maria de Lourdes Pires Bianchi |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Genome instability medicine.medical_specialty Mitotic index Anemia Mitosis Anemia Sickle Cell Biology medicine.disease_cause Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Basal (phylogenetics) hemic and lymphatic diseases Internal medicine medicine Humans Lymphocytes Risk factor Chromosome Aberrations Hematology General Medicine medicine.disease Mutagenesis Mutation Immunology Female Cisplatin Carcinogenesis Oxidative stress |
| Zdroj: | Clinical and Experimental Medicine. 8:31-35 |
| ISSN: | 1591-9528 1591-8890 |
| DOI: | 10.1007/s10238-008-0153-3 |
| Popis: | Sickle cell disease (SCD) is an inherited disorder caused by a single nucleotide substitution in the beta-globin gene. The clinical heterogeneity observed in SCD patients has been attributed to environmental and genetic factors. The patients are subjected to increased oxidative stress, particularly during vaso-occlusive crises and acute chest pain. Another possible cause of oxidative stress in SCD is the high concentration of iron in the patients' plasma. The increase in oxidative stress could be a relevant risk factor for mutagenesis and carcinogenesis. Studies on the frequency of basal chromosomal aberrations in cultured lymphocytes from SCD patients have not been reported so far. In order to contribute to the understanding of the role of the different biomarkers and their relationship with the extremely variable clinical manifestation of SCD, we investigated the frequency of chromosome damage in peripheral lymphocytes from sickle cells patients and healthy controls. We found an increased frequency of chromosome damage and percentage of aberrant metaphases in these patients when compared with control subjects, even at basal values (p0.05). In the cytogenetic sensitivity assay, the results showed that these patients presented a marked decrease in the mitotic index values compared with healthy controls. Cisplatin-induced chromosomal damage in lymphocytes from these patients was significantly higher than the frequency measured in healthy controls. The results obtained in the present study showed that more investigations are needed in order to elucidate the susceptibility to genomic instability of SCD patients. |
| Databáze: | OpenAIRE |
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