Measurement of morphology thickness and refractive index in a melanoma A375 cell line using digital holographic microscopy
| Autor: | María del Socorro Hernández-Montes, J. M. Flores-Moreno, Natalith Palacios-Ortega, Fernando Mendoza-Santoyo |
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| Rok vydání: | 2021 |
| Předmět: |
Morphology (linguistics)
Materials science Holography Phase (waves) 01 natural sciences law.invention 010309 optics Optics law Cell Line Tumor 0103 physical sciences Humans Electrical and Electronic Engineering Engineering (miscellaneous) Cell Shape Melanoma Cell Size Total internal reflection Microscopy business.industry Optical Imaging Signal Processing Computer-Assisted Holographic interferometry Atomic and Molecular Physics and Optics Refractometry Tilt (optics) Digital holographic microscopy business Refractive index |
| Zdroj: | Applied optics. 60(4) |
| ISSN: | 1539-4522 |
| Popis: | Digital holographic microscopy (DHM) is a technique that has high potential for analyzing biological samples and has been successfully applied to the study of cells and cell lines providing information about important parameters such as refractive index, morphology, and dry mass, among others; it has also found applicability to study the effects of therapeutic treatments. Finding the size and shape of cells is important since they tend to change in the presence of some pathologies. In this research work, we obtain the morphology thickness and refractive index of the A375 melanoma cell line through a slight tilting of the cell in a DHM setup. Further, the development of a novel mathematical expression based on this tilt and in the optical phase difference is presented. We show images of melanoma cells with the refractive index information included, and their morphology thickness as rendered from the holographic phase maps recorded with DHM. |
| Databáze: | OpenAIRE |
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