Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro
| Autor: | Bernd-Joachim Benecke, Ronald Reichel |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cytoplasm
Five-prime cap RNA-dependent RNA polymerase chemistry.chemical_compound RNA Transfer Transcription (biology) RNA polymerase Genetics RNA polymerase I Humans RNA Neoplasm Polymerase Cell Nucleus biology Heparin RNA Molecular biology Molecular Weight Kinetics Biochemistry chemistry biology.protein Electrophoresis Polyacrylamide Gel Small nuclear RNA HeLa Cells Research Article |
| Zdroj: | Nucleic Acids Research. 8:225-234 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Isolated HeLa cell nuclei were used to synthesize low molecular weight RNA species in-vitro. The labelled RNA released from the nuclei during the incubation mainly consists of 5S RNA, pre-tRNA and small cytoplasmic RNA species K and L. All these low molecular weight RNA species are synthesized by RNA polymerase C (or III). The polyanion heparin was applied to study the reinitiation of these RNA molecules in-vitro. A comparison of the kinetics of RNA synthesis in the absence and in the presence of this inhibitor demonstrates a highly efficient in-vitro reinitiation of scRNA species K and L as well as 5S and pre-tRNA by RNA polymerase C. These results indicate a general competence of this enzyme to catalyze the de-novo formation of specific gene products in-vitro. |
| Databáze: | OpenAIRE |
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