Sensation of abdominal pain induced by peritoneal carcinomatosis is accompanied by changes in the expression of substance P and μ-opioid receptors in the spinal cord of mice
Autor: | Motohiro Matoba, Minoru Narita, Sadayoshi Furuta, Masami Suzuki, Kazuyoshi Yanagihara, Yasuhito Uezono, Tsutomu Suzuki, Fumiko Chiwaki, Kanako Miyano, Maho Ashikawa, Hiroki Sasaki, Minami Hasegawa, Takahiro Ochiya, Tomoyuki Kawamata |
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Rok vydání: | 2012 |
Předmět: |
medicine.medical_specialty
Abdominal pain Receptor expression Calcitonin Gene-Related Peptide Receptors Opioid mu Substance P Stimulation Mice SCID Real-Time Polymerase Chain Reaction Cell Line chemistry.chemical_compound Mice Internal medicine Cell Line Tumor medicine Animals Humans Luciferases Inflammation Mice Inbred ICR Behavior Animal business.industry Carcinoma Glyceraldehyde-3-Phosphate Dehydrogenases Spinal cord Immunohistochemistry Abdominal Pain Anesthesiology and Pain Medicine Endocrinology Nociception medicine.anatomical_structure Opioid chemistry Pancreatitis Spinal Cord Anesthesia Abdomen medicine.symptom business Proto-Oncogene Proteins c-fos Neoplasm Transplantation medicine.drug |
Zdroj: | Anesthesiology. 117(4) |
ISSN: | 1528-1175 |
Popis: | Background Patients with peritoneal carcinomatosis often report abdominal pain, which is relatively refractory to morphine. It has been considered that a new animal model is required to investigate the mechanism of abdominal pain for the development of optimal treatments for this type of pain. Methods To prepare a peritoneal carcinomatosis model, highly peritoneal-seeding gastric cancer cells, 60As6, were implanted into the abdominal cavity. The nociceptive modality for pain-related behavior was assessed in terms of withdrawal behavior in response to mechanical stimuli and hunching behavior. Tissue samples from mouse dorsal root ganglia and spinal cord were subject to immunohistochemistry and real-time reverse transcription polymerase chain reaction. Results Mice with peritoneal dissemination showed significant hypersensitivity of the abdomen to mechanical stimulation and spontaneous visceral pain-related behavior. There was a significant increase in c-Fos-positive cells in the spinal cord in tumor-bearing mice. Those mice exhibited a remarkable increase in substance P-positive neurons in the dorsal root ganglia (control vs. tumor, 15.4 ± 1.1 vs. 24.2 ± 3.6, P < 0.05, n = 3). A significant decreases in μ-opioid receptor expression mainly in substance P-positive neurons was observed in tumor-bearing mice (69.3 ± 4.9 vs. 38.7 ± 0.9, P < 0.05, n = 3), and a relatively higher dose of morphine was required to significantly reverse the abdominal hypersensitivity. Conclusion Both the up-regulation of substance P and down-regulation of μ-opioid receptor seen in the dorsal root ganglia may be, at least in part, responsible for the abdominal pain-like state associated with peritoneal carcinomatosis. |
Databáze: | OpenAIRE |
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