Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE-UHPLC-MS/MS
| Autor: | Daqiang Yin, You-yu Liu, Xialin Hu, Yi-fan Bao |
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| Rok vydání: | 2017 |
| Předmět: |
Internal standard
Calibration curve Filtration and Separation 010501 environmental sciences Tandem mass spectrometry 01 natural sciences Sensitivity and Specificity Analytical Chemistry Plasma Limit of Detection Tandem Mass Spectrometry Animals Ultrasonics Muscle Skeletal Chromatography High Pressure Liquid 0105 earth and related environmental sciences Detection limit Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Solid Phase Extraction Fishes Reproducibility of Results 0104 chemical sciences Calibration Linear Models Solvents Fish Ultrasonic sensor Algorithms Water Pollutants Chemical |
| Zdroj: | Journal of separation science. 41(10) |
| ISSN: | 1615-9314 |
| Popis: | A confirmatory method for the simultaneous detection of 29 pharmaceuticals in fish muscle and plasma was developed by using solid-phase extraction combined with ultra-high performance liquid chromatography and tandem mass spectrometry. Fish samples were extracted with methanol and enriched using Oasis HLB solid-phase extraction columns in one step. Twenty-nine target pharmaceuticals were quantified by the internal standard method and the calibration curves showed good linearity in a wide range with determination coefficients of greater than 0.913. The detection limits of the pharmaceuticals ranged from 0.01 to 2.00 μg/kg (μg/L). The applicability of the method was checked by precision and recovery experiments. The average recoveries of the 29 pharmaceuticals were between 61 and 111%, and all the relative standard deviations were below 25%. Our reported method has been demonstrated to be sensitive, convenient, rapid, and reliable for the simultaneous determination of 29 pharmaceuticals in fish muscle and plasma. Real sample determination showed that 25 and 9 of the 29 compounds were detected in fish muscle and plasma, respectively. |
| Databáze: | OpenAIRE |
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