A monoclonal antibody that specifically binds chitosan in vitro and in situ on fungal cell walls
| Autor: | Max Schubert, Helga Schinkel, Siham Agdour, Rainer Fischer, Yvonne Olbrich, Stefan Schillberg |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
N-acetyl-D-glucosamine
medicine.drug_class D-glucosamine IgM macromolecular substances Polysaccharide Monoclonal antibody Applied Microbiology and Biotechnology Chitosan Cell wall Mice chemistry.chemical_compound Chitin Cell Wall Glucosamine medicine Animals chemistry.chemical_classification Mice Inbred BALB C biology Fungi technology industry and agriculture Antibodies Monoclonal General Medicine equipment and supplies Antibodies Bacterial Molecular biology Staining carbohydrates (lipids) degree of acetylation (DA) chemistry Biochemistry polysaccharide biology.protein Antibody Biotechnology |
| Popis: | We report the generation of the first monoclonal antibody that specifically binds to the polysaccharide chitosan. Mice were immunized with a mixture of chitosans, and hybridoma clones were screened for specific binders, resulting in the isolation of a single clone secreting a chitosan-specific IgM, mAbG7. In ELISAs, the antibody could bind to chitosans of varying composition, but demonstrated the highest affinity for chitosans with lower degrees of acetylation (DA) and very poor binding to chitin. We tested the ability of the antibody to bind to chitosan in situ, using preparations of fungal cell walls. Immunofluorescence microscopy confirmed that the antibody bound strongly to the cell walls of fungi with high levels of chitosan, whereas poor staining was observed in those species with cell walls of predominantly chitin or cellulose. The potential use of this antibody for the detection of fungal contamination and the protection of plants against fungal pathogens is discussed. |
| Databáze: | OpenAIRE |
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