Synthesis, characterization, and biodistribution of multiple 89Zr-labeled pore-expanded mesoporous silica nanoparticles for PET
| Autor: | Larissa Miller, Barbara Witulla, Christoph Solbach, Sven N. Reske, Mika Lindén, Gordon Winter, Benjamin Baur |
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| Rok vydání: | 2014 |
| Předmět: |
Biodistribution
Materials science Silicon dioxide Transplantation Heterologous Nanoparticle Nanotechnology Mice SCID chemistry.chemical_compound Mice In vivo Animals General Materials Science Chelation Tissue Distribution Radiochemistry Mesoporous silica Silicon Dioxide chemistry Covalent bond Isotope Labeling Positron-Emission Tomography Nanoparticles Zirconium Radiopharmaceuticals Tomography X-Ray Computed Porosity Preclinical imaging |
| Zdroj: | Nanoscale. 6(9) |
| ISSN: | 2040-3372 |
| Popis: | Functional nanoparticles are highly interesting imaging agents for positron emission tomography (PET) due to the possibility of multiple incorporation of positron emitting radionuclides thus increasing the signal strength. Furthermore, long-term nanoparticle biodistribution tests with increased signal-to-noise ratio can be achieved with nanoparticles carrying long-lived isotopes. Mesoporous silica nanoparticles, MSNs, have recently attracted a lot of interest as both imaging agents and carriers for drugs in vitro and in vivo. Here we present results related to the synthesis of PET imageable MSNs carrying the long-lived (89)Zr isotope (half-life of 78.4 hours). Here, (89)Zr(4+) was immobilized through covalent attachment of the complexing agent p-isothiocyanatobenzyldesferrioxamine (DFO-NCS) to large-pore MSNs. Due to the presence of the high DFO content on the MSNs, quantitative (89)Zr(4+) labeling was achieved within just a few minutes, and no subsequent purification step was needed in order to remove non-complexed (89)Zr(4+). The stability of the (89)Zr-labeled MSNs against leaching of (89)Zr(4+) was verified for 24 hours. The high signal strength of the (89)Zr-DFO-MSNs was evidenced by successful PET imaging using a mouse model at particle loadings one order of magnitude lower than those previously applied in PET-MSN studies. The biodistribution followed the same trends as previously observed for MSNs of different sizes and surface functionalities. Taken together, our results suggest that (89)Zr-DFO-MSNs are promising PET imaging agents for long-term in vivo imaging. |
| Databáze: | OpenAIRE |
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