Generation and characterization of three new monoclonal antibodies detecting the allospecificities HLA-A2, w69, HLA-A3 and HLA-B13
| Autor: | Fischer M, A Raghavachar, Ballas M, A. Wölpl, Thomas Eiermann, Shraga F. Goldmann |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.drug_class
Immunology Human leukocyte antigen HLA-A3 Antigen In Vitro Techniques Biology Monoclonal antibody Biochemistry Peripheral blood mononuclear cell Mice Antigen HLA-A3 HLA Antigens HLA-A2 Antigen Genetics medicine HLA-B Antigens Animals Humans Immunology and Allergy HLA-B13 Antigen Mice Inbred BALB C Leukemia medicine.diagnostic_test Antibodies Monoclonal General Medicine Virology Molecular biology Acute Disease biology.protein Antibody Tissue typing |
| Zdroj: | Tissue Antigens. 29:201-207 |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1987.tb01577.x |
| Popis: | Immunization of balb/c mice with peripheral blood mononuclear cells of a leukemic patient possessing the antigens HLA-A2,3; B7,35 resulted in the polymorphic monoclonal antibody (moab) UL-101/68 (IgM) defining HLA-A3. Immunization of a second group of balb/c mice with the lymphoblastoid cell line BER homozygous for HLA-A2; B13 revealed two polymorphic moabs UL-39/10 (IgG3) specific for HLA-B13 and UL-39/24 (IgG2b) defining HLA-A2,w69. Immunoprecipitation and polyacrylamide gel electrophoresis with the two moabs of the IgG isotype confirmed the class I structure of the recognized antigens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text