Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines
| Autor: | Deborah de Almeida Bauer Guimarães, Otniel Freitas-Silva, Ayelén D Nigra, Anderson Junger Teodoro, César G. Prucca, Germán A. Gil |
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| Přispěvatelé: | AYELÉN D. NIGRA, Universidad Nacional de Córdoba, DEBORAH DE ALMEIDA BAUER GUIMARÃES, Universidade Federal do Estado do Rio de Janeiro -UNIRIO, CÉSAR G. PRUCCA, Universidad Nacional de Córdoba, OTNIEL FREITAS SILVA, CTAA, ANDERSON J. TEODORO, Universidade Federal do Estado do Rio de Janeiro - UNIRIO, GERMÁN A. GIL, Universidad Nacional de Córdoba. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Aging Article Subject Population Cell Culture Techniques Apoptosis Breast Neoplasms Coffea Coffea canephora Biochemistry Coffee Food technology 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor medicine Humans Viability assay education Clonogenic assay skin and connective tissue diseases education.field_of_study biology QH573-671 Plant Extracts Extrato Vegetal Cancer Cell Biology General Medicine Cell cycle biology.organism_classification medicine.disease 030104 developmental biology Grão 030220 oncology & carcinogenesis Café Robusta Cancer research Produto de Origem Vegetal Female Câncer Cytology Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing thedevelopment and progression of cancer. However, there is still controversy around these claims. As indicated in our previousworks, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientificevidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability,proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability wasevaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, andfunctionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantlyreduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced inMDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extractinduces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, andtriggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity wasdemonstrated in both 2D and 3D culture cell models Made available in DSpace on 2021-06-15T12:01:04Z (GMT). No. of bitstreams: 1 Antitumor-Effects-of-Freeze-Dried-Robusta-Coffee-Coffea-canephora-Extracts-on-Breast-Cancer-Cell-Lines.pdf: 2995444 bytes, checksum: 691c655dde88cf18d7904d63b257457f (MD5) Previous issue date: 2021 Research Article. |
| Databáze: | OpenAIRE |
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