Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study
| Autor: | Chantal Medina, Marie-Laure Stachowicz, Jean-Christophe Fricain, Davit Hakobyan, Hugo Oliveira, Julie Guillermet-Guibert, Nathalie Dusserre, C. Handschin |
|---|---|
| Přispěvatelé: | Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie du système nerveux central - Institut François Magendie, Université Bordeaux Segalen - Bordeaux 2-IFR8-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Chassande, Olivier, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cellular pathology
Carcinogenesis Swine Ductal cells [SDV]Life Sciences [q-bio] Biomedical Engineering Bioengineering Acinar Cells Biochemistry Cell Line law.invention Biomaterials 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine law Spheroids Cellular Pancreatic cancer medicine Animals 030304 developmental biology 0303 health sciences 3D bioprinting business.industry Lasers Transdifferentiation Bioprinting Cancer General Medicine medicine.disease Pancreas Exocrine Rats 3. Good health ErbB Receptors Pancreatic Neoplasms [SDV] Life Sciences [q-bio] Ki-67 Antigen medicine.anatomical_structure 030220 oncology & carcinogenesis Cell Transdifferentiation Printing Three-Dimensional Cancer research Gelatin Methacrylates Pancreatitis Pancreas business Biotechnology |
| Zdroj: | Biofabrication Biofabrication, IOP Publishing, 2020, 12 (3), pp.035001. ⟨10.1088/1758-5090/ab7cb8⟩ Biofabrication, 2020, 12 (3), pp.035001. ⟨10.1088/1758-5090/ab7cb8⟩ |
| ISSN: | 1758-5082 1758-5090 |
| DOI: | 10.1088/1758-5090/ab7cb8⟩ |
| Popis: | International audience; Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model should prove useful for the study of the internal and external factors that contribute to the formation of precursor PDAC lesions and to cancer progression, and may therefore shed light on future PDAC therapy strategies. |
| Databáze: | OpenAIRE |
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