Circumventing the Crabtree effect in cell culture
| Autor: | Michèle J. C. de Kok, Rob C. I. Wüst, Alexander F. Schaapherder, Jan H.N. Lindeman, Jaap A. Bakker, Melissa C. Zuiderwijk, Sylvia E. Le Dévédec |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Metabolic reprogramming Cell Culture Techniques Cell Biology Mitochondrion Biology Mitochondrial Dynamics Culture Media Mitochondria 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Glucose Metabolism Cell culture Molecular Medicine Crabtree effect Humans Oxidative phosphorylation Molecular Biology Neuroscience Glycolysis 030217 neurology & neurosurgery |
| Zdroj: | de Kok, M J C, Schaapherder, A F, Wüst, R C I, Zuiderwijk, M, Bakker, J A, Lindeman, J H N & Le Dévédec, S E 2021, ' Circumventing the Crabtree effect in cell culture : A systematic review ', Mitochondrion, vol. 59, pp. 83-95 . https://doi.org/10.1016/j.mito.2021.03.014 Mitochondrion, 59, 83-95. ELSEVIER SCI LTD Mitochondrion, 59, 83-95. Elsevier BV |
| ISSN: | 1567-7249 |
| Popis: | Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from aoxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect. |
| Databáze: | OpenAIRE |
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