Intussusceptive pillar formation in developing porcine glomeruli
| Autor: | Tim Vandecasteele, Ward De Spiegelaere, Wim Van Den Broeck, Ingrid Walter, Pieter Cornillie, Anastasia Logothetidou, Waltraud Tschulenk |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endothelium Physiology Angiogenesis Organogenesis Kidney Glomerulus Sus scrofa Neovascularization Physiologic Gestational Age 030226 pharmacology & pharmacy ANGIOGENESIS law.invention MECHANISMS Extracellular matrix 03 medical and health sciences 0302 clinical medicine KIDNEY Microscopy Electron Transmission INFLAMMATION law medicine Animals Intussusceptive angiogenesis RECONSTRUCTION Chemistry MICROVASCULAR GROWTH Pillar Endothelial Cells Biology and Life Sciences Metanephros VASCULAR MORPHOGENESIS Cell biology Glomerular capillary Capillaries Extracellular Matrix MODEL 030104 developmental biology medicine.anatomical_structure CELLS Glomerulus sense organs Electron microscope Cardiology and Cardiovascular Medicine MATRIX Metanephric kidney Intraluminal pillar |
| Zdroj: | JOURNAL OF VASCULAR RESEARCH |
| ISSN: | 1018-1172 1423-0135 |
| Popis: | Background/Aims: Intussusceptive angiogenesis (IA) is a dynamic process which contributes to vascular expansion and remodeling. Intraluminal pillars have long been the distinctive structural indicator of IA. However, the mechanism of their formation has not been fully elucidated. Methods: Using light and electron microscopy, we studied intussusceptive vascular growth in the developing porcine metanephric kidney. Results: We observed intraluminal pillars formed by endothelial cells in the vasculature of developing glomeruli. Their diameter was < 2.5 µm, consistent with the diameter of nascent pillars. TEM revealed that the majority of these pillars consisted only of endothelium. However, a central core of extracellular matrix (ECM) covered by endothelium, reminiscent of a more mature intussusceptive pillar, was also found in the lumen of a glomerular capillary. Perivascular cells or pericytes were not involved in the pillar structure during these stages of formation. Conclusion: This study shows ECM presence in a mature intussusceptive pillar without any perivascular cell involvement in the structure. This leads to the hypothesis that ECM deposition precedes the participation of these cells in the formation of intraluminal pillars during IA in porcine metanephric glomerular capillaries. |
| Databáze: | OpenAIRE |
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