Dual Lactate Clearance in the Viability Assessment of Livers Donated After Circulatory Death With Ex Situ Normothermic Machine Perfusion
| Autor: | Jun-Kyu Shin, Ola Ahmed, Gundumi A. Upadhya, Fangyu Zhou, Kathleen Byrnes, Yiing Lin, Brian W. Wong, William C. Chapman, Jae-Sung Kim, Min Xu, Lucy V. Randle, Yuehui Zhu |
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| Rok vydání: | 2021 |
| Předmět: |
Transplantation
Machine perfusion Necrosis RD1-811 Glycogen Kinase business.industry Circulatory death Andrology chemistry.chemical_compound chemistry Downregulation and upregulation Organ Donation and Procurement ComputingMethodologies_DOCUMENTANDTEXTPROCESSING medicine Extracellular Surgery medicine.symptom business Immunostaining |
| Zdroj: | Transplantation Direct Transplantation Direct, Vol 7, Iss 12, p e789 (2021) |
| ISSN: | 2373-8731 |
| DOI: | 10.1097/txd.0000000000001243 |
| Popis: | Supplemental Digital Content is available in the text. Background. Perfusate lactate clearance (LC) is considered one of the useful indicators of liver viability assessment during normothermic machine perfusion (NMP); however, the applicable scope and potential mechanisms of LC remain poorly defined in the setting of liver donation after circulatory death. Methods. The ex situ NMP of end-ischemic human livers was performed using the OrganOx Metra device. We further studied the extracellular signal-regulated kinases (phospho-extracellular signal-regulated kinase1/2 [pERK1/2]) pathway and several clinical parameters of these livers with successful LC (sLC, n = 5) compared with non-sLC (nLC, n = 5) in the perfusate ( |
| Databáze: | OpenAIRE |
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