Regulation of arginase expression by T-helper II cytokines and isoproterenol
| Autor: | Bernard R. Boulanger, Gloria Gellin, Paul A. Kearney, Andrew R. Barksdale, Betty J. Tsuei, Juan B. Ochoa, Andrew C. Bernard, Mary E. Maley |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Arginine medicine.medical_treatment Blotting Western 8-Bromo Cyclic Adenosine Monophosphate Nitric oxide Cell Line chemistry.chemical_compound Th2 Cells Transforming Growth Factor beta Internal medicine medicine Animals Interleukin-13 biology Arginase Reverse Transcriptase Polymerase Chain Reaction Macrophages Isoproterenol Interleukin Drug Synergism Adrenergic beta-Agonists Interleukin-10 Nitric oxide synthase Blot Cytokine Endocrinology chemistry Enzyme Induction biology.protein Cytokines Surgery Interleukin-4 Transforming growth factor |
| Zdroj: | Surgery. 135(5) |
| ISSN: | 0039-6060 |
| Popis: | Background Trauma causes a release of catecholamines, transforming growth factor–β (TGF-β), and T-helper II cytokines (TH2). Individually, these substances also induce arginase in macrophages. The purpose of this study was to determine the synergistic interactions between isoproterenol, TGF-β, and TH2 cytokines on arginase expression in macrophages. Methods Confluent RAW 264.7 macrophages were incubated with various combinations of interleukins 4, 10, and 13 (IL-4, IL-10, IL-13), and TGF-β with isoproterenol over 48 hours. Arginase activity, as well as arginase I expression by Western blot and reverse transcriptase–polymerase chain reaction, were measured. Results Although isoproterenol, IL-4, IL-10, and IL-13 individually induced arginase, significant synergy between the combination of isoproterenol with either TGF-β or the TH2 cytokines was observed. All cytokines except IL-10 also induced arginase I protein and mRNA. Arginase II protein was detected in cells exposed to IL-10. Conclusions We conclude that isoproterenol synergizes with IL-4, IL-13, and TGF-β to increase arginase I mRNA and protein, as well as arginase activity in RAW 264.7 macrophages. Further, IL-10 synergizes with isoproterenol to increase arginase activity and arginase II protein. These synergistic mechanisms may compete with nitric oxide synthase for l -arginine substrate, thus shunting away available arginine from nitric oxide production and contributing to cellular immunosuppression observed after trauma. |
| Databáze: | OpenAIRE |
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