Distribution of insulin-like growth factor-I mRNA in the mandibular condyle and rib cartilage of the rat during growth

Autor: Vivian Visnapuu, S Syrjänen, O. Rönning, Timo Peltomäki
Rok vydání: 2002
Předmět:
Zdroj: Archives of Oral Biology. 47:791-798
ISSN: 0003-9969
DOI: 10.1016/s0003-9969(02)00115-2
Popis: This study makes a molecular biological comparison of primary and secondary cartilage at an early phase of postnatal development. The distribution of insulin-like growth factor-I (IGF-I) mRNA expression in the mandibular condyle and rib cartilage of 1-28-day-old rats was examined after in situ hybridisation using an oligo probe cocktail for IGF-I mRNA. In the condyle, expression was localised to a narrow strip under the articular layer where the cells are undifferentiated. Essentially, no differences were found in IGF-I synthesis within three samples from the same age group or between different age groups. In rib cartilage, IGF-I mRNA was localised within the germinative, proliferative and early hypertrophic cell layers in 1-28-day-old rats. Again, there were no differences in expression among animals of the same age or as a function of age. This pattern of IGF-I mRNA expression indicates that IGF-I synthesis during growth of the mandibular condylar cartilage is different from that of costal cartilage. The findings shed light on the problem of overgrowth often associated with the use of costochondral grafts to replace defective mandibular condyles.
Databáze: OpenAIRE
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