MRI monitoring of transplanted neonatal porcine islets labeled with polyvinylpyrrolidone‐coated superparamagnetic iron oxide nanoparticles in a mouse model
| Autor: | Eric Boivin, Adnan Black, Kieran Purich, Anthony G. Tessier, Ryan W. Hung, Ray V. Rajotte, Haolei Cai, Ping Wu, Yulian Wu, Dong Xin, B. G. Fallone, Bin Yang, Zhihao Xu, Bao-You Xu, Bo Zhang, Gina R. Rayat |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Pathology medicine.medical_specialty Swine Xenotransplantation medicine.medical_treatment Transplantation Heterologous Immunology Islets of Langerhans Transplantation Spleen Diabetes Mellitus Experimental Islets of Langerhans Mice medicine Animals Humans Transplantation Kidney geography Type 1 diabetes geography.geographical_feature_category medicine.diagnostic_test business.industry technology industry and agriculture Povidone Magnetic resonance imaging Islet medicine.disease Magnetic Resonance Imaging In vitro medicine.anatomical_structure Magnetic Iron Oxide Nanoparticles business |
| Zdroj: | Xenotransplantation. 29 |
| ISSN: | 1399-3089 0908-665X |
| Popis: | Islet transplantation is a potential treatment option for certain patients with type 1 diabetes; however, it still faces barriers to widespread use, including the lack of tools to monitor islet grafts post-transplantation. This study investigates whether labeling neonatal porcine islets (NPI) with polyvinylpyrrolidone-coated superparamagnetic iron oxide nanoparticles (PVP-SPIO) affects their function, and whether this nanoparticle can be utilized to monitor NPI xenografts with magnetic resonance imaging (MRI) in a mouse model. In vitro, PVP-SPIO-labeled NPI in an agarose gel was visualized clearly by MRI. PVP-SPIO-labeled islets were then transplanted under the kidney capsules of immunodeficient nondiabetic and diabetic mice. All diabetic mice that received transplantation of PVP-SPIO-labeled islets reached normoglycemia. Grafts appeared as hypo-intense areas on MRI and were distinguishable from the surrounding tissues. Following injection of spleen cells from immunocompetent mice, normoglycemic recipient mice became diabetic and islet grafts showed an increase in volume, accompanied by a mixed signal on MRI. Overall, this study demonstrates that PVP-SPIO did not affect the function of NPI that PVP-SPIO-labeled islets were easily seen on MRI, and changes in MRI signals following rejection suggest a potential use of PVP-SPIO-labeled islets to monitor graft viability. |
| Databáze: | OpenAIRE |
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