Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype
| Autor: | Thomas J. Borell, Benjamin Alderete, Arie Perry, Bernd W. Scheithauer, Judith R. O'Fallon, Allan J. Yates, Justin S. Smith, David W. Kimmel, Yuriko A. Minn, Sandra M. Hosek, Robert B. Jenkins, Peter C. Burger, Burt G. Feuerstein, Gayatry Mohapatra |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Tumor suppressor gene Oligodendroglioma Loss of Heterozygosity In situ hybridization Astrocytoma Biology Loss of heterozygosity Gene mapping Chromosome 19 Glioma Genetics medicine Humans neoplasms Molecular Biology In Situ Hybridization Fluorescence Sequence Deletion medicine.diagnostic_test Brain Neoplasms medicine.disease nervous system diseases Chromosomes Human Pair 1 Chromosomes Human Pair 19 Comparative genomic hybridization Fluorescence in situ hybridization |
| Zdroj: | Oncogene. 18:4144-4152 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1202759 |
| Popis: | Allelic alterations of chromosomes 1 and 19 are frequent events in human diffuse gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas (Cairncross et al., 1998; Smith et al., submitted). Using 115 human diffuse gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coefficients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were defined on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18% of astrocytomas (10/55) and in 73% (24/33) of oligodendrogliomas (P < 0.0001), and loss of the 19q13.3 region was found in 38% (21/55) of astrocytomas and 73% (24/33) of oligodendrogliomas (P = 0.0017). Loss of both regions was found in 11% (6/55) of astrocytomas and in 64% (21/33) of oligodendrogliomas (P < 0.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers. |
| Databáze: | OpenAIRE |
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