Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse
| Autor: | Miriam O. Ribeiro, Bruna Pascarelli Pedrico do Nascimento, Cynthia Rodrigues Muller, Carmen Lucia Penteado Lancellotti, Felipe Henriques, Talita Sayuri Higa, Miguel L. Batista, Anna Laura Viacava Américo, Antonio C. Bianco, Nailliw Z. Preite, Fabiana S. Evangelista |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Beta-3 adrenergic receptor medicine.medical_specialty Sympathetic nervous system Adrenergic receptor Adipose Tissue White Lipolysis Endocrinology Diabetes and Metabolism White adipose tissue Diet High-Fat Article Mice Norepinephrine 03 medical and health sciences chemistry.chemical_compound Endocrinology Adipose Tissue Brown Internal medicine Adipocyte Brown adipose tissue medicine Animals Obesity Adiposity TECIDO ADIPOSO Mice Knockout business.industry Thermogenesis Lipid Metabolism Cold Temperature 030104 developmental biology medicine.anatomical_structure chemistry Receptors Adrenergic beta-3 business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | The brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through β-adrenergic receptors (AR). Here, we wished to define the role played by the ARβ3isoform in this process. This study focused on the ARβ3knockout mice (ARβ3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). ARβ3KO mice defend core temperature during cold exposure (4°C for 5 h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, ARβ3KO mice kept on a high-fat diet (HFD; 40% fat) for 8 weeks exhibited greater susceptibility to diet-induced obesity, markedly increased epididymal adipocyte area with clear signs of inflammation. The HFD-induced glucose intolerance was similar in both groups but serum hypertriglyceridemia and hypercholesterolemia were less intense in ARβ3KO animals when compared with WT controls. Isoproterenol-induced lipolysis in isolated white adipocytes as assessed by glycerol release was significantly impaired in ARβ3KO animals despite normal expression of key proteins involved in lipid metabolism. In conclusion, ARβ3inactivation does not affect BAT thermogenesis but increases susceptibility to diet-induced obesity by dampening WAT lipolytic response to adrenergic stimulation. |
| Databáze: | OpenAIRE |
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