Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets
| Autor: | Maria I. Wanderley, Luiz F. Rezende, André Otávio Peres Protzek, Marcus A.F. Corat, Fabiano Ferreira, Rafael Ludemann Camargo, Bruno Rodrigo da Silva Lippo, Vanessa Cristina Arantes, Everardo M. Carneiro, Almir Gonçalves Wanderley, Daniel P. Udrisar, Thiago M. Batista, Ana Paula Gameiro Cappelli, Renato Chaves Souto Branco, Helena Sampaio |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry In Vitro Techniques Biology Biochemistry Islets of Langerhans chemistry.chemical_compound Internal medicine Diet Protein-Restricted medicine Animals Cyclic adenosine monophosphate Rats Wistar Protein kinase A Molecular Biology Protein Kinase C Protein kinase C Nutrition and Dietetics Insulin Pancreatic islets Cyclic AMP-Dependent Protein Kinases Rats Crosstalk (biology) Endocrinology medicine.anatomical_structure chemistry Phosphorylation Signal transduction Signal Transduction |
| Zdroj: | The Journal of Nutritional Biochemistry. 26:556-562 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2014.12.010 |
| Popis: | Protein restriction in the early stages of life can result in several changes in pancreatic function. These alterations include documented reductions in insulin secretion and in cytoplasmic calcium concentration [Ca2+]i. However, the mechanisms underlying these changes have not been completely elucidated and may result, in part, from alterations in signaling pathways that potentiate insulin secretion in the presence of glucose. Our findings suggest that protein restriction disrupts the insulin secretory synergism between Cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and Ca2+-dependent protein kinase C (PKC) in isolated islets. Western blot analysis demonstrated reduced levels of both phospho-cAMP response element-binding protein (phospho-CREB) at Ser-133 and substrates phosphorylated by PKCs (Phospho-(Ser) PKC substrate), suggesting that PKA and PKC activity was impaired in islets from rats fed a low-protein diet (LP). cAMP levels and global Ca2+ entry were also reduced in LP islets. In summary, our findings showed that protein restriction altered the crosstalk between PKA and PKC signaling pathways, resulting in the alteration of secretory synergism in isolated islets. |
| Databáze: | OpenAIRE |
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