Updates on the Production of Therapeutic Antibodies Using Human Hybridoma Technique
| Autor: | Alexei A. Ivanov, Xenia A. Glukhova, G. V. Afanas'eva, Marco Avila-Rodriguez, Michael M. Zaripov, George E. Barreto, Gjumrakch Aliev, Julia A. Trizna, Olga V. Prusakova, Igor P. Beletsky, Rimma A. Poltavtseva, Anatoly S. Glukhov |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pharmacology Hybridomas biology Computer science Biomedical Technology Antibodies Monoclonal Mice Transgenic Computational biology Mice transgenic 03 medical and health sciences Mice 030104 developmental biology Human hybridoma Antibodies monoclonal Drug Design Drug Discovery Therapeutic antibody Immunology biology.protein Animals Humans Antibody Biomedical technology |
| Zdroj: | Current pharmaceutical design. 22(7) |
| ISSN: | 1873-4286 |
| Popis: | Therapeutic antibodies are implicated into the very promising and fast growing area of pharmaceutics. Human hybridoma technology, allowing generation of natural human antibodies in a native form, seems to be the most direct way that require no additional modifications for production of therapeutic antibodies. However, technical difficulties in human hybridoma creation discovered in the 80s of the last century have switched the mainstream therapeutic antibody development into new directions like display and transgenic mice techniques. These approaches have provided remarkable achievements in antibody engineering within last 15 years, but also revealed other limitations. Thus, it is time to turn back to forgotten human hybridoma technology. In this review, we describe new advances in all components of human hybridoma technology and discuss challenges in generating novel therapeutic mABs based on hybridoma technologies. |
| Databáze: | OpenAIRE |
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