No impact of soluble epoxide hydrolase rs4149243, rs2234914 and rs751142 genetic variants on the development of type II diabetes and its hypertensive complication among Jordanian patients
| Autor: | Su-Jun Lee, Maysoon Khamees, Yazun Jarrar, Tariq Al-Qirim, Ma'mon M. Hatmal, Ismail S. Mahmoud, Walhan Alshaer |
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| Rok vydání: | 2021 |
| Předmět: |
Epoxide hydrolase 2
Genotype 030204 cardiovascular system & hematology Polymorphism Single Nucleotide law.invention 03 medical and health sciences 0302 clinical medicine law medicine Humans Genetic Predisposition to Disease 030212 general & internal medicine Allele Allele frequency Polymerase chain reaction Epoxide Hydrolases Genetics Jordan medicine.diagnostic_test business.industry Haplotype General Medicine genomic DNA Diabetes Mellitus Type 2 Haplotypes Lipid profile business |
| Zdroj: | International Journal of Clinical Practice. 75 |
| ISSN: | 1742-1241 1368-5031 |
| DOI: | 10.1111/ijcp.14036 |
| Popis: | Background Human soluble epoxide hydrolase plays a major role in cardiovascular homeostasis. Genetic variants in the EPHX2 gene among different ethnic groups are associated with cardiovascular complications, such as hypertension. However, no reports regarding the association of EPHX2 genotype with hypertension among type II diabetic (T2D) patients of Middle Eastern Jordanian origin exist. Objective The current study aimed to elucidate the association of the EPHX2 allele, genotype, and haplotype with T2D, hypertension, and parameters of lipid profile parameters among Jordanian T2D patients. Methods Ninety-three genomic DNA samples of non-diabetic controls and 97 samples from T2D patients were genotyped for EPHX2 rs4149243, rs2234914, and rs751142 genetic variants. The DNA samples were amplified using polymerase chain reaction (PCR) and then sequenced using Applied Biosystems Model (ABI3730x1). The functionality of intronic EPHX2 variants was predicted using the in silico Berkely Drosophila Genome Project software. Results We found no significant (P > 0.05) association between the EPHX2 rs4149243, rs2234914, and rs751142 allele, genotype, and haplotype and the incidence of T2D and hypertension. Additionally, no association (P > 0.05) between these EPHX2 genetic variants with the baseline total cholesterol, low- and high-density lipoproteins, and triglycerides among both non-diabetic and diabetic volunteers was found. However, we found an inter-ethnic variation (χ2 -test, p value ˂ 0.05) in the allele frequency of the EPHX2 rs4149243 and rs2234914 variants between Jordanians and other ethnic populations. Also, the in silico Berkely Drosophila Genome Project software predicted that the intronic EPHX2 rs4149243 could alter the splicing of intron 7. Conclusions It can be concluded from this study that EPHX2 rs4149243, rs2234914, and rs751142 genetic variants do not play a role in the development of T2D and hypertension among Jordanian T2D patients. Further genetic studies with larger sample sizes are needed to find out the association of other functional EPHX2 variants with cardiovascular diseases among T2D patients in Jordan. |
| Databáze: | OpenAIRE |
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