Lipoteichoic acid induces delayed myocardial protection in isolated rat hearts: a comparison with endotoxin
| Autor: | Guenther Oprea, Paula A. Zacharowski, Jan Mersmann, Kai Zacharowski, Patrick Schober, Antje Nebert |
|---|---|
| Přispěvatelé: | Anesthesiology, Other Research |
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Male Resuscitation Lipopolysaccharide Neutrophils Ischemia Anti-Inflammatory Agents Myocardial Infarction Emergency Nursing Pharmacology Dexamethasone chemistry.chemical_compound Intensive care Medicine Animals cardiovascular diseases Myocardial infarction Rats Wistar business.industry medicine.disease Systemic Inflammatory Response Syndrome Rats Endotoxins Teichoic Acids stomatognathic diseases Disease Models Animal chemistry Anesthesia Ischemic Preconditioning Myocardial Emergency Medicine lipids (amino acids peptides and proteins) Lipoteichoic acid Cardiology and Cardiovascular Medicine business Ex vivo medicine.drug |
| Zdroj: | Resuscitation, 79(2), 311-315. Elsevier Ireland Ltd Schober, P, Oprea, G, Mersmann, J, Nebert, A, Zacharowski, K & Zacharowski, P A 2008, ' Lipoteichoic acid induces delayed myocardial protection in isolated rat hearts: a comparison with endotoxin ', Resuscitation, vol. 79, no. 2, pp. 311-315 . https://doi.org/10.1016/j.resuscitation.2008.06.001 |
| ISSN: | 0300-9572 |
| DOI: | 10.1016/j.resuscitation.2008.06.001 |
| Popis: | Summary Objective Preconditioning with bacterial wall fragments lipopolysaccharide (LPS) or lipoteichoic acid (LTA) reduce myocardial infarct size after ischaemia and reperfusion (I/R) in rats. Preconditioning with LTA reduces neutrophil accumulation during reperfusion and thereby ameliorates one of myocardial reperfusion injury's most important mechanisms. In this study, we use an ex vivo model of regional myocardial I/R to investigate LTA versus LPS induced preconditioning in a system devoid of leukocytes. Methods Rats were subjected to LTA or LPS with or without dexamethasone pre-treatment. Twenty-four hours after LTA or LPS challenge, hearts were removed and retrogradely perfused in a Langendorff set-up. Hearts underwent 20 min of regional ischaemia followed by 2 h of reperfusion. Ischaemic preconditioning (IPC) was performed as a positive control. Myocardial infarct size was determined as the primary end-point. Results LTA and LPS preconditioning both lead to a marked reduction in infarct size similar to IPC, however, no significant differences were found between LTA and LPS. The reduction in infarct size was abrogated by dexamethasone pre-treatment. Conclusion We conclude that preconditioning with LTA and likewise with LPS confers myocardial protection in an ex vivo setting devoid of leukocytes. Dexamethasone inhibits preconditioning, suggesting that the underlying mechanism is dependent upon induction of a systemic inflammatory response to a LTA or LPS stimulus. |
| Databáze: | OpenAIRE |
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