Effects of storage media, supplements and cryopreservation methods on quality of stem cells
| Autor: | Özgür Doğuş Erol, Mehmet Emin Seker, Fatima Aerts-Kaya, Burcu Pervin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Histology
Cryobiology Stromal cell Chemistry Mesenchymal stem cell Cell Biology Review Dimethyl sulfoxide Cryopreservation Cell biology Haematopoiesis Induced pluripotent stem cells Cell culture Genetics Cryoprotective agents Stem cell Induced pluripotent stem cell Mesenchymal stromal/stem cells Molecular Biology Genetics (clinical) Hematopoietic stem cells |
| Zdroj: | World Journal of Stem Cells |
| ISSN: | 1948-0210 |
| Popis: | Despite a vast amount of different methods, protocols and cryoprotective agents (CPA), stem cells are often frozen using standard protocols that have been optimized for use with cell lines, rather than with stem cells. Relatively few comparative studies have been performed to assess the effects of cryopreservation methods on these stem cells. Dimethyl sulfoxide (DMSO) has been a key agent for the development of cryobiology and has been used universally for cryopreservation. However, the use of DMSO has been associated with in vitro and in vivo toxicity and has been shown to affect many cellular processes due to changes in DNA methylation and dysregulation of gene expression. Despite studies showing that DMSO may affect cell characteristics, DMSO remains the CPA of choice, both in a research setting and in the clinics. However, numerous alternatives to DMSO have been shown to hold promise for use as a CPA and include albumin, trehalose, sucrose, ethylene glycol, polyethylene glycol and many more. Here, we will discuss the use, advantages and disadvantages of these CPAs for cryopreservation of different types of stem cells, including hematopoietic stem cells, mesenchymal stromal/stem cells and induced pluripotent stem cells. |
| Databáze: | OpenAIRE |
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