Cell attachment area of rat mesenchymal stem cells correlates with their osteogenic differentiation level on substrates without osteoconductive property
| Autor: | Atsuo Ito, Atsushi Yamazaki, Mayu Yasunaga, Masayuki Kakehata, Hidehiko Yashiro, Motohiro Hirose, Shogo Hashimoto |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Osteocalcin Cell Cell Culture Techniques Biophysics Cell morphology Biochemistry Cell-Matrix Junctions 03 medical and health sciences 0302 clinical medicine Osteogenesis medicine Animals Secretion Molecular Biology Cells Cultured biology Chemistry Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Tissue culture polystyrene Alkaline Phosphatase Rats Cell biology Durapatite 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Polystyrenes Zirconium Negative correlation Periodic microstructure |
| Zdroj: | Biochemical and Biophysical Research Communications. 525:1081-1086 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.03.013 |
| Popis: | Cell morphology is related to proliferation and differentiation. We previously reported that cell attachment area of rat mesenchymal stem cells (MSCs) is negatively correlated with their osteogenic differentiation level on osteoconductive hydroxyapatite (HAp) with various microstructures. In this study, the correlation between the cell attachment area and osteogenic differentiation level was investigated on substrates without osteoconductive property using tissue culture polystyrene (TCPS), and 3 mol% yttria-stabilized zirconia (3Y-TZP) with or without surface periodic microstructures. It was found that the osteogenic differentiation level after 3 weeks of culture increased with a decrease in cell attachment area after 3 h of culture. The square of the correlation coefficient between cell attachment area and osteocalcin secretion content was 0.845 among the three types of substrates. Thus, the negative correlation between cell attachment area and differentiation level is confirmed even when cultured on substrates without osteoconductive property. These findings suggest that the correlation between the cell attachment area of rat MSCs and osteogenic differentiation level could also apply to various types of substrate, regardless of osteoconductive property. |
| Databáze: | OpenAIRE |
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