Human adipose-Derived Mesenchymal Stem Cells Improve Motor Functions and are Neuroprotective in the 6-Hydroxydopamine-Rat Model for Parkinson’s Disease when Cultured in Monolayer Cultures but Suppress Hippocampal Neurogenesis and Hippocampal Memory Function when Cultured in Spheroids
| Autor: | Barbara Steiner, Jennifer Altschüler, Andreas Kurtz, Jürgen Berg, Manfred Roch, Anne Schwerk, Christine Winter |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Cancer Research Tyrosine 3-Monooxygenase Neurogenesis Transplantation Heterologous Cell Culture Techniques Substantia nigra Motor Activity Hippocampal formation Biology Mesenchymal Stem Cell Transplantation Hippocampus Parkinsonian Disorders Memory Neurotrophic factors Glial Fibrillary Acidic Protein Animals Humans Rats Wistar Oxidopamine Cells Cultured Hydroxydopamine Microscopy Confocal Glial fibrillary acidic protein Reverse Transcriptase Polymerase Chain Reaction Brain-Derived Neurotrophic Factor Dentate gyrus Cell Differentiation Mesenchymal Stem Cells Cell Biology Anatomy Neuroprotection Cell biology Transplantation Disease Models Animal Adipose Tissue nervous system biology.protein Female |
| Zdroj: | Stem Cell Reviews and Reports. 11:133-149 |
| ISSN: | 1558-6804 1550-8943 |
| DOI: | 10.1007/s12015-014-9551-y |
| Popis: | Adult human adipose-derived mesenchymal stem cells (MSC) have been reported to induce neuroprotective effects in models for Parkinson's disease (PD). However, these effects strongly depend on the most optimal application of the transplant. In the present study we compared monolayer-cultured (aMSC) and spheroid (sMSC) MSC following transplantation into the substantia nigra (SN) of 6-OHDA lesioned rats regarding effects on the local microenvironment, degeneration of dopaminergic neurons, neurogenesis in the hippocampal DG as well as motor and memory function in the 6-OHDA-rat model for PD. aMSC transplantation significantly increased tyrosine hydroxylase (TH) and brain-derived neurotrophic factor (BDNF) levels in the SN, increased the levels of the glial fibrillary acidic protein (GFAP) and improved motor functions compared to untreated and sMSC treated animals. In contrast, sMSC grafting induced an increased local microgliosis, decreased TH levels in the SN and reduced numbers of newly generated cells in the dentate gyrus (DG) without yet affecting hippocampal learning and memory function. We conclude that the neuroprotective potential of adipose-derived MSC in the rat model of PD crucially depends on the applied cellular phenotype. |
| Databáze: | OpenAIRE |
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