Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress
| Autor: | Janelle M. Ryals, Angela N. Pierce, Olivia C. Eller, Julie A. Christianson, Elizabeth R. Di Silvestro, Ruipeng Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hippocampus chemistry.chemical_compound Transient Receptor Potential Channels 0302 clinical medicine Corticosterone Mast Cells TRPA1 Cation Channel media_common Urinary bladder Maternal Deprivation General Neuroscience Urinary Bladder Diseases Interstitial cystitis Interleukin 10 medicine.anatomical_structure Cytokines Intercellular Signaling Peptides and Proteins Female Urinary bladder disease Hypothalamic–pituitary–adrenal axis medicine.medical_specialty Colon media_common.quotation_subject Urinary Bladder TRPV1 Urination Article 03 medical and health sciences Internal medicine medicine Animals Receptor PAR-2 RNA Messenger Molecular Biology business.industry Rectum medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology Animals Newborn chemistry Neurology (clinical) business Stress Psychological 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1639:58-73 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2016.02.039 |
| Popis: | Early adverse events have been shown to increase the incidence of interstitial cystitis/painful bladder syndrome in adulthood. Despite high clinical relevance and reports of stress-related symptom exacerbation, animal models investigating the contribution of early life stress to female urological pain are lacking. We examined the impact of neonatal maternal separation (NMS) on bladder sensitivity and visceral neuroimmune status both prior-to, and following, water avoidance stress (WAS) in adult female mice. The visceromotor response to urinary bladder distension was increased at baseline and 8d post-WAS in NMS mice, while colorectal sensitivity was transiently increased 1d post-WAS only in naïve mice. Bladder micturition rate and output, but not fecal output, were also significantly increased following WAS in NMS mice. Changes in gene expression involved in regulating the stress response system were observed at baseline and following WAS in NMS mice, and WAS reduced serum corticosterone levels. Cytokine and growth factor mRNA levels in the bladder, and to a lesser extent in the colon, were significantly impacted by NMS and WAS. Peripheral mRNA levels of stress-responsive receptors were differentially influenced by early life and adult stress in bladder, but not colon, of naïve and NMS mice. Histological evidence of mast cell degranulation was increased in NMS bladder, while protein levels of protease activated receptor 2 (PAR2) and transient receptor potential ankyrin 1 (TRPA1) were increased by WAS. Together, this study provides new insight into mechanisms contributing to stress associated symptom onset or exacerbation in patients exposed to early life stress. |
| Databáze: | OpenAIRE |
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