The nucleotide-binding oligomerization domain-containing-2 ligand muramyl dipeptide enhances phagocytosis and intracellular killing of Escherichia coli K1 by Toll-like receptor agonists in microglial cells
| Autor: | Sandra Schütze, Tommy Regen, Uwe-Karsten Hanisch, Roland Nau, Sandra Ribes, Angela Borisch, Sandra Redlich, Nina Adam, Hana Janova |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cytotoxicity
Immunologic Phagocytosis Immunology Antigen presentation Nod2 Signaling Adaptor Protein Biology Ligands Microbiology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Adjuvants Immunologic NOD2 medicine Escherichia coli Immunology and Allergy Animals Cells Cultured Escherichia coli Infections 030304 developmental biology 0303 health sciences Toll-like receptor Microglia Toll-Like Receptors Ligand (biochemistry) Flow Cytometry Immunity Innate 3. Good health Cell biology Mice Inbred C57BL medicine.anatomical_structure Neurology chemistry Neurology (clinical) Acetylmuramyl-Alanyl-Isoglutamine 030217 neurology & neurosurgery Muramyl dipeptide Intracellular |
| Zdroj: | Journal of neuroimmunology. 252(1-2) |
| ISSN: | 1872-8421 |
| Popis: | Increasing the phagocytic activity of microglia could improve the resistance of immunocompromised patients to CNS infections. We studied the microglial responses upon stimulation with the Nod2 ligand muramyl dipeptide (MDP) alone or in combination with a TLR1/2, 3 or 4 agonist. MDP caused a mild release of NO, but induced neither a significant release of pro-inflammatory cytokines nor an expression of molecules associated with professional antigen presentation. Using the Escherichia coli K1 model, microglial pre-stimulation with MDP enhanced bacterial phagocytosis which was strengthened on TLR-pre-stimulated cells. Dual pre-stimulation of Nod2 and TLR1/2 or 4 caused maximal phagocytosis and intracellular killing. |
| Databáze: | OpenAIRE |
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