NK-CELL STIMULATING PROPERTIES OF A MEMBRANE PROTEOGLYCANE FROM NON-CAPSULATED KLEBSIELLA PNEUMONIAE BIOTYPE a
| Autor: | Anne-Marie Pinel, Hans Wigzell, Lucien Dussourd D'hinterland, Urban Ramstedt, Gerard Normier |
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| Rok vydání: | 2009 |
| Předmět: |
Cytotoxicity
Immunologic Male Cellular immunity Time Factors Klebsiella pneumoniae Immunology Mice Inbred Strains Microbiology Natural killer cell Mice Adjuvants Immunologic In vivo medicine Animals Dose-Response Relationship Drug General Immunology and Microbiology biology Biological activity General Medicine biology.organism_classification Enterobacteriaceae In vitro Killer Cells Natural Bacterial vaccine medicine.anatomical_structure Interferon Type I Chromatography Gel Female Proteoglycans Injections Intraperitoneal Spleen Bacterial Outer Membrane Proteins |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Series C: Immunology. :233-243 |
| ISSN: | 0108-0202 |
| Popis: | Upon testing the individual fractions of a composite bacterial vaccine for biological activities, a potent immuno-stimulatory capacity could be demonstrated within a crude membrane proteoglycan preparation from Klebsiella pneumoniae. One of its characteristic features was the capacity to induce alpha-type interferon and increased NK activity in vivo in mice, following intraperitoneal or oral administration. A highly purified fraction from the crude preparation was obtained using alkaline hydrolysis, delipidation and size fractionation. This fraction was shown to be a very potent inducer of NK cells in vivo or in vitro, where in the latter systems concentrations as low as 0.1 microgramme per ml were highly efficient. |
| Databáze: | OpenAIRE |
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