The Human Plasma Kinin System II. Contact Activation of Plasma Prekallikrein and Factor XI in Factor XII-Deficient Plasma
| Autor: | Ozge-Anwar Ah, Movat Hz, J. G Scott |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Density gradient
Arginine Factor XI Deficiency Size-exclusion chromatography Vascular permeability General Biochemistry Genetics and Molecular Biology Centrifugation Density Gradient Methods Humans Factor XI Chromatography Chemistry Kallikrein Blood Coagulation Disorders Kinin Chromatography Ion Exchange Electrophoresis Disc Blood Coagulation Factors Molecular Weight Clotting time Sephadex Factor XII Chromatography Gel Kallikreins Blood Coagulation Tests gamma-Globulins circulatory and respiratory physiology |
| Zdroj: | Experimental Biology and Medicine. 138:330-337 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-138-35890 |
| Popis: | SummaryNoncontacted factor XII-deficient human plasma was mixed with Celite, the protein adsorbed to the Celite was eluted with a concentrated salt solution, and then chromatographed on QAE-Sephadex A-50, followed by Sephadex G-200.The first (excluded) peak obtained by anion exchange chromatography contained substances with physicochemical and biological properties indistinguishable from plasma kallikrein and “contact activation product.” Material obtained with the excluded peak enhanced vascular permeability, hydrolyzed arginine ester, liberated kinin from both fresh and heated plasma, corrected the prolonged clotting time of plasmas congenitally deficient in factors XII or XI, artificially depleted of XI, but did not enhance the clotting of plasmas deficient in factors IX, VIII, and X. On gel filtration the molecular weight of kallikrein was estimated to be about 90, 000 and that of the clot-promoting material was about 170,000. The latter was confirmed by sucrose density gradient ultracentrifugation.We... |
| Databáze: | OpenAIRE |
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