Semi-permeable microcapsules for cell culture: Ultra-structural characterization
| Autor: | D. Hopwood, A. Cushieri, E. L. Newman, Sami M. Shimi |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Alginates
Globular protein Analytical chemistry Pharmaceutical Science Capsules Bioengineering Permeability law.invention Colloid and Surface Chemistry law Humans Polylysine Semipermeable membrane Physical and Theoretical Chemistry Cells Cultured chemistry.chemical_classification Organic Chemistry Molecular Weight Microscopy Electron Membrane chemistry Chemical engineering Transmission electron microscopy Cell culture Permeability (electromagnetism) Ultrastructure Electron microscope |
| Zdroj: | Journal of Microencapsulation. 8:307-316 |
| ISSN: | 1464-5246 0265-2048 |
| DOI: | 10.3109/02652049109069557 |
| Popis: | Microcapsules made from alginate-poly(L-lysine)-alginate membranes have been studied as vehicles for cell culture in a number of laboratories. We have examined their permeability, robustness and ultrastructure in detail. Permeability to globular proteins could be controlled by using poly-lysine of different mean MW in their construction. However, this parameter also affected the degree to which microencapsulated living cells leaked out of the capsules during and after preparation. Poly-lysine of low MW produced a relatively permeable and robust membrane whereas a high MW produced capsules with the reverse characteristics. A MW of 22,000 appears to be optimal in forming robust capsules which are relatively impermeable to high MW species such as immunoglobulins. The structure of the semipermeable membrane was investigated by electron microscopy and found to be complex but entirely consistent with the data on protein permeability and cell leakage. Microcapsules were not disrupted by gentle treatment with trypsin or chelating agents but dissolved with the addition of heparin, sodium dodecyl sulphate or sodium hydroxide. Empty microcapsules implanted into the peritoneal cavity of rats elicited a host cellular reaction but remained intact for at least three months. |
| Databáze: | OpenAIRE |
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