Resolution of 1,2-epoxyhexane by Rhodotorula glutinis using a two-phase membrane bioreactor
| Autor: | Won Jae Choi, J.A.M. de Bont, C.A.G.M. Weijers, Chunho Choi |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Aqueous solution
Chromatography Dodecane Aqueous two-phase system Rhodotorula General Medicine Membrane bioreactor Applied Microbiology and Biotechnology Solvent chemistry.chemical_compound Industrial Microbiology Bioreactors chemistry Industriële microbiologie Organic chemistry Racemic mixture Life Science Epoxy Compounds Solubility Epoxide hydrolase Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology 53 (1999) Applied Microbiology and Biotechnology, 53, 7-11 |
| ISSN: | 0175-7598 |
| Popis: | Large-scale resolution of epoxides by the yeast Rhodotorula glutinis was demonstrated in an aqueous/organic two-phase cascade membrane bioreactor. Due to the chemical instability and low solubility of epoxides in aqueous phases, an organic solvent was introduced into the reaction mixture in order to enhance the resolution of epoxide. A cascade hollow-fiber membrane bioreactor was used (1) to minimize the toxicity of organic solvents towards the epoxide hydrolase of R. glutinis, and (2) to remove inhibitory amounts of formed diol from the yeast cell containing aqueous phase. Dodecane was selected as a suitable solvent and 1,2-epoxyhexane as a model substrate. By use of this membrane bioreactor, highly concentrated (0.9 M in dodecane) enantiopure (> 98% ee) (S)-1,2-epoxyhexane (6.5 g, 30% yield) was obtained from the racemic mixture. |
| Databáze: | OpenAIRE |
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